揭开cuproptosis在肺纤维化病原和预后中的作用:一个整合性的单细胞转录组学和微阵列分析
Mengting Chen1, Jialu Chen2, Jiaxiang Zhang1
1Department of Critical Care Medicine, School of Medicine, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, 201620, China.
Molecular and cellular biochemistry
|March 13, 2026
概括
铜,一种依赖铜的细胞死亡,与肺纤维化 (PF) 的进展相反相关. 这项研究确定了关键基因,并开发了PF的预后模型,提供了新的治疗见解.
科学领域:
- 细胞生物学 细胞生物学
- 肺部医学 肺部医学
- 计算生物学 计算生物学
背景情况:
- 肺纤维化 (PF) 是一种渐进的肺部疾病,其原因尚不清楚,治疗选择有限.
- cuproptosis 是一种依赖铜的细胞死亡途径,正在成为 PF 病原发生的潜在因素.
- 了解cuproptosis的作用对于开发新的PF疗法至关重要.
研究的目的:
- 为了研究cuproptosis在肺纤维化中的生物学作用.
- 根据与cuproptosis相关的基因 (CRG) 开发PF的预后模型.
- 探索 cuproptosis 和纤维化过程之间的关系.
主要方法:
- 从小鼠模型和人类IPF患者的单细胞和大量RNA测序数据的综合分析.
- 使用LASSO和考克斯回归识别CRG.
- 构建和验证一个4-CRG预后签名.
- 使用小鼠模型,细胞检测和人类IPF标本进行实验验证.
主要成果:
- 开发了一种新的4-CRG特征 (LIAS,LIPT1,ATP7A,PDHB) 来分层IPF患者.
- 高风险患者的生存率较低,细胞外基质和脂质代谢增加.
- 在纤维化肺组织和细胞中,CRG显著下调.
- 在纤维化的环境中观察到细胞活力受损和cuproptosis标志物的增加.
结论:
- 质亡与PF的进展相反相关,这表明它起着保护作用.
- 开发的4-CRG签名作为一个强大的工具,用于预测PF的临床预后.
- 这项研究为纤维生成中的铜介导机制和潜在的治疗点提供了新的见解.
相关概念视频
01:20COPD: Pathogenesis and Clinical Features
2.1K
Chronic obstructive pulmonary disease (COPD) is a group of lung conditions that progressively worsen over time, including chronic bronchitis and emphysema. This cluster of diseases collectively leads to a gradual and irreversible decline in lung function over time.
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...
2.1K
01:30Pulmonary Hypertension: Classification and Pathogenesis
766
Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...
There are various classifications for PH, each relating to different underlying causes and also...
766
01:20Chronic Obstructive Pulmonary Disease-II: Pathophysiology
5.0K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Chronic Inflammation
5.0K
01:27Pneumothorax-II
1.3K
Pneumothorax is a medical condition defined by the buildup of air in the pleural space between the lungs and the chest wall. This accumulation of air can lead to partial or complete lung collapse, resulting in a range of clinical manifestations. Understanding the clinical presentation and effective management strategies is crucial for healthcare professionals in providing timely and appropriate care to individuals with pneumothorax.
Clinical Manifestations:
Clinical Manifestations:
1.3K
01:25Chronic Obstructive Pulmonary Disease-III: Symptoms and Complications.
4.0K
Understanding the variety of primary symptoms and systemic complications that characterize chronic obstructive pulmonary disease (COPD) is crucial for healthcare professionals.
Symptoms of COPD can be classified as primary or systemic. Primary symptoms relate to reduced airflow, while systemic or extrapulmonary symptoms relate to COPD's broader impact on the body.
Primary Symptoms of COPD:
Symptoms of COPD can be classified as primary or systemic. Primary symptoms relate to reduced airflow, while systemic or extrapulmonary symptoms relate to COPD's broader impact on the body.
Primary Symptoms of COPD:
4.0K
01:24Chronic Obstructive Pulmonary Disease
2.9K
COPD is defined as a heterogeneous lung condition marked by persistent respiratory symptoms such as dyspnea, cough, and sputum production, caused by abnormalities in the airways that cause airflow obstruction.
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
Smoking is a primary risk factor for COPD, with over 80% of patients having a history of it. Patients typically experience progressive dyspnea or labored breathing, frequent coughing, and recurrent pulmonary infections. Many eventually succumb to respiratory failure, characterized by...
2.9K