记录视觉地图的协议在老鼠的上结核和视觉皮层与内在的光学成像
Flora Boutet-Porretta1, Josien Visser2, Arthur Matthys1
1Center for Interdisciplinary Research in Biology, Collège de France, CNRS, INSERM, PSL-Neuro, Université PSL, Paris, France; Doctoral School N°158, Sorbonne Université, Paris, France.
STAR protocols
|March 13, 2026
概括
这项研究引入了一种使用内在光学成像 (IOI) 的快速协议,用于绘制小鼠上层结核 (SC) 和视觉皮层 (V1) 中的视觉功能. 该方法可以快速有效地在SC中实现视网膜和定向地图.
科学领域:
- 神经科学是一个神经科学.
- 视觉系统研究 视觉系统研究
- 功能性大脑成像 - 功能性大脑成像
背景情况:
- 内在光学成像 (IOI) 对于绘制小鼠大脑中的视觉功能至关重要.
- 目前用于绘制上层结核 (SC) 和初级视觉皮层 (V1) 的现有方法可能耗时.
- 需要有效的协议来详细分析视觉处理.
研究的目的:
- 在鼠标SC和V1.1.中提出用于内在光学成像 (IOI) 的快速协议.
- 为了能够快速地绘制SC中的视网膜和方向偏好.
- 为了方便在V1.中获取眼界优势图的获取.
主要方法:
- 利用周期性刺激和富里埃分析来绘制SC视网膜和方向偏好.
- 采用独立的单眼刺激来获得V1眼主导图.
- 提供了手术准备,成像设置和数据预处理的详细说明.
主要成果:
- 在15分钟内成功地绘制了小鼠SC中的视网膜和定向偏好.
- 在V1.1中启用了对眼主导图的获取.
- 证明了提交的IOI协议的效率和有效性.
结论:
- 开发的协议提供了一种快速有效的方法,用于在鼠标SC和V1.1中进行视觉功能映射.
- 这种技术大大减少了获得详细视觉地图所需的时间.
- 该协议为神经科学研究视觉处理提供了宝贵的工具.
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