在低收入和中等收入国家使用基于等离子体的熟练测试项目消除yaws的外部质量评估
Claudia Mueller1, Serges Tchatchouang2, Laud Anthony Basing3
1Institute of International Animal Health/One Health, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald, Insel Riems, Germany.
PLoS neglected tropical diseases
|March 13, 2026
概括
一个新的外部质量评估 (EQA) 程序使用基于等离子体的熟练测试项目 (PTIs) 已开发为yaws根除运动. 该计划表现出稳定性和准确性,满足低收入和中等收入国家实验室的需求.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 传染病控制和控制传染病
- 分子生物学分子生物学
背景情况:
- 根除病需要在资源有限的环境中进行可靠的实验室诊断.
- 现有的外部质量评估 (EQA) 计划可能无法充分为低收入和中等收入国家 (LMICs) 服务.
研究的目的:
- 建立一个基于等离子体的EQA程序,用于yaws根除.
- 满足LMIC中参考和地区实验室的诊断需求.
主要方法:
- 熟练测试项目 (PTIs) 设计使用Treponema pallidum (TP) 和Haemophilus ducreyi (HD) 的等离子体.
- 在各种环境条件下评估了PTI的储存稳定性.
- 一组PTIs分发给加纳,科特迪瓦和喀麦隆的实验室进行定量实时PCR (qPCR) 和循环介导同热放大 (LAMP) 测试.
- 现场样本被重新测试以验证实验室性能.
主要成果:
- 基于等离子体的PTI在干燥条件下显示稳定性,没有显著的副本数损失.
- 参考实验室在qPCR方面取得了很高的一致性 (97.7%).
- 地区实验室显示LAMP (76.0-100.0%) 的可变一致性,在检测低水平的HD DNA方面存在挑战.
- 现场样本的重新测试证实了非洲参考实验室对TP和HD的100%正确识别.
结论:
- 一个功能性的,基于等离子体的EQA程序成功地为资源贫困的热带环境开发.
- 该计划证明适合于yaws根除运动,并可以作为其他疾病控制计划的典范.
相关概念视频
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