Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

79
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
79

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Advancements in Protein-Based Therapeutic Delivery Approaches Targeting the Blood-Brain Barrier and Insights on Computational Strategies.

Critical reviews in therapeutic drug carrier systems·2025
Same author

Predicting phase-I metabolism of piceatannol: an in silico study.

In silico pharmacology·2024
Same author

Structural dynamics and catalytic modulations of Aβ regulating enzymes as future outlook for Alzheimer's.

Biochemical and biophysical research communications·2022
Same author

Design, Synthesis, and Biological Evaluation of 2-(2-Bromo-3-nitrophenyl)-5-phenyl-1,3,4-oxadiazole Derivatives as Possible Anti-Breast Cancer Agents.

Chemistry & biodiversity·2020
Same author

Cytotoxicity and Synergistic Effect of Biogenically Synthesized Ternary Therapeutic Nano Conjugates Comprising Plant Active Principle, Silver and Anticancer Drug on MDA-MB-453 Breast Cancer Cell Line.

Asian Pacific journal of cancer prevention : APJCP·2020
Same author

Corrigendum: Design, Synthesis, and Biological Evaluation of (E)-N'-((1-Chloro-3,4-Dihydronaphthalen-2-yl)Methylene)Benzohydrazide Derivatives as Anti-prostate Cancer Agents.

Frontiers in chemistry·2019

相关实验视频

Updated: Mar 15, 2026

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
13:46

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

6.3K

基于分子动力学和生物物理验证的辅助剂选择用于BDNF立方体组合的配方.

Radhakrishnan Nithya1, Muthiah Ramanathan2

  • 1Department of Pharmaceutics, PSG College of Pharmacy, Coimbatore, Tamil Nadu, India.

Journal of molecular graphics & modelling
|March 13, 2026
PubMed
概括

这项研究优化了脑衍生神经营养因子 (BDNF) 配方,使用计算方法选择辅助剂. 鉴定出植物醇,Tween 80和PEG 200可以增强BDNF稳定性和药物输送的生物可用性.

关键词:
减弱的总反射 里叶变换红外光谱学 (ATR-FTIR)这就是为什么BDNF是BDNF.立方体体是一个立方体体.分子动力学 (MD) 模拟蛋白辅助剂的相互作用

更多相关视频

Formulating and Characterizing an Exosome-based Dopamine Carrier System
06:08

Formulating and Characterizing an Exosome-based Dopamine Carrier System

Published on: April 4, 2022

3.8K
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
10:53

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Published on: September 15, 2014

15.8K

相关实验视频

Last Updated: Mar 15, 2026

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
13:46

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

6.3K
Formulating and Characterizing an Exosome-based Dopamine Carrier System
06:08

Formulating and Characterizing an Exosome-based Dopamine Carrier System

Published on: April 4, 2022

3.8K
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
10:53

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Published on: September 15, 2014

15.8K

科学领域:

  • 生物化学和制药科学 生物化学和制药科学
  • 计算化学计算化学
  • 药物输送系统 药物输送系统

背景情况:

  • 治疗性蛋白质配方,如来自大脑的神经营养因子 (BDNF),面临着由于不稳定性和生物可用性差的挑战,特别是用于中枢神经系统的输送.
  • 开发稳定和有效的基于蛋白质的疗法需要仔细选择辅料,以保持蛋白质的完整性.

研究的目的:

  • 开发一个集成的计算-实验工作流程,以合理选择 BDNF 加载的立方体配方中的辅助剂.
  • 为了确定最佳的脂质,稳定剂和水,以提高BDNF的稳定性和生物可用性.

主要方法:

  • 基于结构的计算分析,包括SiteMap,分子对接和分子动力学 (MD) 模拟,以评估蛋白质与辅助剂的相互作用.
  • 选各种脂质,稳定剂和水基物与BDNF的分子兼容性.
  • 实验验证使用减弱总反射率-里埃变换红外光谱 (ATR-FTIR) 来确认蛋白质二次结构的保存.

主要成果:

  • 植物醇与BDNF表现出有利的极性和疏水性相互作用,被确定为最佳脂质.
  • 分别选择了80和PEG 200作为首选的稳定剂和水.
  • 模拟MD显示过渡但稳定蛋白质辅助剂接触,保持BDNF的结构完整性. ATR-FTIR证实了BDNF的二次结构的保存.

结论:

  • 该研究成功地展示了用于选择辅助剂的预测计算策略,以优化神经热素配方.
  • 鉴定的辅助剂组合 (植物醇,Tween 80,PEG 200) 增强了BDNF的稳定性和结构完整性.
  • 这种方法为辅助剂-蛋白质相互作用提供了有价值的分子层面的见解,指导开发稳定的治疗性蛋白质输送系统.