橄-蓝藻-细菌微藻-细菌颗粒污泥用于减轻毒素风险:培养,特征和形成机制
Bingdang Wu1, Yu Zhai1, Shiqi Li1
1School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China; Jiangsu Province Engineering Research Center of Water Resilient Cities, Suzhou, 215009, China; Jiangsu Provincial Key Laboratory of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China.
Journal of environmental management
|March 13, 2026
概括
在微藻-细菌颗粒污泥 (MBGS) 废水处理中,Oocystis borgei的添加有效地抑制了蓝藻. 这种方法减少了毒素,并通过增强细菌细胞外聚合物质来促进粒度.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 废水处理工程 废水处理工程
背景情况:
- 微藻-细菌颗粒污泥 (MBGS) 提供优质的营养物质去除和碳中和的废水处理.
- 在MBGS中蓝藻细菌的流行引起了人们对毒素生产的担忧.
- 开发藻-菌MBGS对于安全高效的废水处理至关重要.
研究的目的:
- 用Oocystis borgei (O.borgei) 作为注射剂来培养橄-蓝藻细菌的MBGS.
- 调查O.borgei对蓝藻细菌丰富度,毒素水平和颗粒化机制的影响.
- 了解微藻-细菌相互作用在细胞外聚合物质 (EPS) 生产和MBGS形成中的作用.
主要方法:
- 使用O.borgei注射剂培养MBGS.
- 流细胞计和16S/18SrRNA基因测序用于微生物社区分析.
- 基于自身光的细胞分类技术 (AFCS) 用于细胞外蛋白质 (PN) 分析.
- 甲基因组分析以确定与EPS分泌相关的基因.
- 基因组注释以确定占主导地位的细菌类.
主要成果:
- 实现了持续的低水平的蓝菌和显著降低的蓝毒素度 (0.15μg/g-VSS).
- 在微藻-细菌混合培养 (MBMC) 中,O.borgei的添加导致细菌PN含量和整体EPS显著增加.
- 关键的EPS分泌基因 (lolD,lptB) 在颗粒化过程中呈现出更多的丰富性,特别是在蛋白质细菌中.
- 编码EPS产生基因的蛋白质细菌主导了藻-蓝藻细菌的MBGS.
结论:
- 囊 (Oocystis borgei) 有效地培养藻-菌MBGS,确保低菌和毒素水平.
- 微藻 (O.borgei) 刺激蛋白质细菌增强PN分泌,促进EPS的产生和MBGS的颗粒化.
- 这项研究为藻-菌MBGS培养提供了可行的策略,并为其工程应用提供了机械洞察力.
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