多阶段合成微生物联盟通过重塑新陈代谢和介导无氧消化过程中的功能稳定性权衡,优于单阶段增强
Miaomiao An1, Jiarui Yu1, Xianghao Lin1
1Beijing Key Laboratory of Food Processing and Safety in Forestry, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Bioresource technology
|March 13, 2026
概括
工程微生物群落促进了食物浪费中的甲生产. 与单阶段联盟相比,多阶段合成联盟 (SynCom-YSJ) 显著增加了甲产量和减少了抑制性化合物.
科学领域:
- 微生物学 微生物学
- 环境生物技术 环境生物技术
- 生物化学工程 生物化学工程
背景情况:
- 食品废弃物无氧消化 (AD) 面临着诸如低甲产量和挥发性脂肪酸 (VFA) 积累等挑战,原因是微生物社区的不平衡.
- 合成微生物群落 (SynComs) 提供了一种通过引入特定微生物功能来提高AD性能的策略.
- 使用SynComs的生物增量可能可以克服本地微生物群的局限性,以改善生物气生产.
研究的目的:
- 构建和评估两个功能驱动的SynComs (仅含甲的SynCom-J和多阶段的SynCom-YSJ) 用于食品废物的生物增量.
- 将SynCom-YSJ和SynCom-J在半连续反应器中的性能与本地微生物组进行比较.
- 研究SynCom生物增量对微生物群落结构,代谢活动和AD过程参数的影响.
主要方法:
- 构建两个SynComs:SynCom-J (仅含有甲) 和SynCom-YSJ (含水解,酸性,甲性阶段).
- 使用SynCom-J和SynCom-YSJ使用原生微生物组的半连续反应器的生物增量.
- 每天用部分化食品废弃物的原料进行养.
- 监测甲产量,VFA概况和微生物社区动态 (甲基因组学).
主要成果:
- 与对照组相比,SynCom-YSJ显著增加了22%的甲产量,超过了SynCom-J (8%的增长).
- 这两种SynComs都使酸盐积累减少了1.6倍,几乎消除了启动滞后阶段.
- SynCom-YSJ诱导了更广泛的代谢重编程,增强了水解,酸生成和甲基生成途径.
- 在微生物网络稳定性 (SynCom-J) 和工艺性能 (SynCom-YSJ) 之间观察到一个权衡,SynCom-YSJ优先考虑甲产量.
结论:
- 使用SynCom-YSJ的多阶段生物增量有效地提高了食品废弃物AD中的甲产量和工艺稳定性.
- 工程SynComs可以重塑AD微环境,并重新编程微生物新陈代谢以提高效率.
- 本研究提供了一个生态知情的设计原则,用于开发SynComs,以平衡生物技术应用中的性能和弹性.
相关概念视频
01:30Environmental Applications of Microorganisms
1.3K
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
1.3K
00:46Bioremediation
22.7K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
22.7K
01:23Microbial Fermentation
1.8K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.8K
01:18Amino Acid Catabolism
1.5K
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
1.5K