使用发酵气体中的挥发性有机化合物预测酒发酵指标的当前状态
Keisuke Yamauchi1, Fumika Kimura1, Masahiro Furuno1
1Department of Biotechnology, Graduate School of Engineering, The University of Osaka, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Journal of bioscience and bioengineering
|March 13, 2026
概括
本研究引入了一种使用发酵气体中的挥发性有机化合物 (VOC) 来实时预测关键代谢物度的非侵入性方法. 这种方法增强了酒等行业的流程监控.
科学领域:
- 生物技术和发酵科学 生物技术和发酵科学
- 分析化学 分析化学
- 过程监控 过程监控
背景情况:
- 微生物发酵对于生产食品,药品和生物能源至关重要.
- 目前的监测依赖于离线代谢物分析,这耗时且限制了实时过程控制.
- 开发非侵入性实时监测方法对于优化发酵产量和产品质量至关重要.
研究的目的:
- 开发一种使用发酵气体中的挥发性有机化合物 (VOC) 预测发酵成分的非侵入性方法.
- 建立VOC档案与主要发酵指标 (如糖,有机酸和乙醇度) 之间的联系.
- 在酒发酵模型系统中验证VOC监测的可行性.
主要方法:
- 在发酵气体中的挥发性有机化合物 (VOC) 的分析使用气体染色学质谱法 (GC-MS) 与Tenax TA.
- 开发多变量回归模型,特别是直角部分最小平方 (OPLS) 回归.
- 发酵气体和的并行采样用于模型校准和验证.
主要成果:
- 开发了高度准确的模型,从VOC档案中预测糖,有机酸和乙醇度.
- 清晰的VOC模式,如法兰和化物在早期,与后期阶段的化合物,如较高的酒精和相反相关.
- 该研究确定了特定的VOC,表明发酵期间的代谢过渡.
结论:
- 挥发性有机化合物 (VOC) 分析提供了一种可行的方法,用于定量,实时预测发酵进展.
- 这种非侵入性方法提供了一种新的方式,将香味化学与发酵中的工业过程控制联系起来.
- 这些发现具有很大的潜力,可以改善各种基于发酵的行业的监测和控制策略.
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