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图书馆准备偏差植物病毒检测:多 (A) mRNA丰富与rRNA枯竭在胡和大中的偏差

Hoseong Choi¹, Dong Woo Kang^2,3, Yeonhwa Jo⁴

¹Biocube System, Inc., Suwon 16648, Republic of Korea.

International journal of molecular sciences

|March 14, 2026

概括

总RNA测序提供了比mRNA测序更全面的植物病毒组分析. 总RNA-seq检测到更多的病毒和完整的基因组,为病毒发现提供了不那么有偏见的观点.

科学领域:

植物病毒学植物病毒学
生物信息学是一种生物信息学.
这是下一代测序.

背景情况:

高通量RNA测序 (RNA-seq) 对于发现植物病毒至关重要.
图书馆准备方法,如核糖体RNA (rRNA) 枯竭和多元A选择,可以在病毒检测中引入偏差.
了解这些偏差对于准确的植物病毒组分析至关重要.

研究的目的:

为了比较rRNA-depleted总RNA-seq和poly(A) 选择的mRNA-seq的疗效,用于植物病毒组分析.
评估图书馆准备方法对病毒检测和基因组重建的影响.
为全面发现植物病毒确定最佳的RNA-seq方法.

主要方法:

现场采集的胡和大样本用于RNA提取.
采用了两种图书馆准备方法:rRNA-depleted总RNA-seq和poly(A) 选择的mRNA-seq.
测序数据分析了病毒序列,结合长度和基因组完整性.

主要成果:

与mRNA-seq相比,缺少rRNA的总RNA-seq始终检测到更多的病毒,更长的病毒结合体和完整的多方DNA病毒基因组.
mRNA-seq偏向于高度表达的多基化病毒,缺少几个病毒和由总RNA-seq.q检测到的病毒基因组段.
总RNA-seq在大中发现了低滴度RNA病毒,可能表明污染,mRNA-seq没有检测到.

结论:

缺少rRNA的总RNA-seq为植物病毒组发现和基因组重建提供了更全面,更少偏见的方法.
mRNA-seq适用于量化多基病毒和分析宿主基因表达,同时进行病毒样本分析.
选择RNA-seq库准备方法显著影响植物病毒体表征的广度和深度.

关键词:

大,大,大,大.转换组学转换组学转换组学胡胡胡胡是一个人类遗传学是个学科.植物病毒组 (virome) 是一种植物病毒组.选择mRNA-seqq的多类A.没有rRNA的总RNA-seqq.

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