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Overview of the Vascular System

Overview of the Vascular System

The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...

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Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein

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典型和多核内皮细胞在低密度脂蛋白暴露下之间的功能差异

Vadim Cherednichenko¹, Diana Kiseleva^1,2, Ulyana Khovantseva^1,3

¹Petrovsky National Research Center of Surgery, 119435 Moscow, Russia.

International journal of molecular sciences

|March 14, 2026

概括

与典型的内皮细胞 (TEC) 相比,多核变异内皮细胞 (MVEC) 显示胆固醇积累和炎症增加. 这些发现表明MVEC可能会加剧慢性内皮功能障碍和动脉样硬化中的血管炎症.

关键词:

它们是内皮细胞.内皮质功能障碍在内皮质功能障碍低密度脂蛋白是一种低密度脂蛋白.多核变体内皮细胞.

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科学领域:

内皮细胞生物学内皮细胞生物学
血管恒温是血管的恒温.
心血管疾病的发病源是心血管疾病的发病源.

背景情况:

内皮细胞功能障碍是动脉样硬化和心血管疾病的核心原因.
多核变异性内皮细胞 (MVECs) 存在于病理性血管区域,但人们对其了解甚少.
了解MVEC的功能对于阐明血管疾病的机制至关重要.

研究的目的:

为了比较MVECs与典型内皮细胞 (TECs) 的脂质处理,炎症激活,屏障功能和分泌特征.
研究低密度脂蛋白 (LDL) 暴露对这些内皮细胞表型的影响.
将MVEC描述为一种独特的内皮细胞表型,有助于血管功能障碍.

主要方法:

MVECs是由EA.hy926细胞的聚乙烯糖醇诱导的融合产生的.
细胞被暴露在LDL中,分析包括胆固醇的生物化学测定,细胞因子的ELISA,基因表达的定量实时PCR,以及秘密的DIA-LC-MS.
免疫细胞化学被用来评估紧结蛋白位址.

主要成果:

与TEC相比,MVEC在暴露于LDL时表现出增强的细胞内胆固醇积累.
MVECs显示基底预炎性标志物表达升高 (IL1B,IL6,NFKB1) 并放大IL6/IL8对LDL的反应.
MVECs显示抗氧化剂,屏障完整性 (TJP1) 和静血性 (VWF) 基因的表达减少,vWF和EDN1的分泌量减少,并改变了ZO-1分布,表明结节不稳定.

结论:

MVECs代表了一种独特的内皮表型,具有增加的脂质积累和持续的促炎激活.
MVECs具有改变的分泌信号,降低了屏障完整性和降低了静血潜力.
这些特征表明,MVECs有助于慢性内皮功能障碍和血管炎症,特别是在高脂血症条件下.