在Lagomorphs的基因组中短间隔元素的C家族:结构,进化,转录和转录多化
Ilia G Ustyantsev1, Sergei A Kosushkin2, Dmitri A Kramerov1
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
Animals : an open access journal from MDPI
|March 14, 2026
概括
短间隔元素 (SINEs) 是移动的遗传元素. 在拉戈莫尔法中,C SINE家族表现出独特的多基解,在子胚胎发育早期活动的特定变体.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 短间隔元素 (SINEs) 是由RNA聚合酶III转录的非自主逆转移子.
- 哺乳动物T+ SINE具有多基化信号,终端器和A丰富的尾巴,使多基化成为可能.
- 拉戈莫尔法中C SINE家族代表了研究SINE进化和功能的一个独特案例.
研究的目的:
- 为了研究基因组架构,进化历史,和C SINE家族的多化在Lagomorpha.
- 为了确定负责C SINE多基解的cis-determinants.
- 在胚胎早期发育过程中确定C SINE的转录活性.
主要方法:
- 生物信息学在科动物和科动物的基因组中搜索C SINE副本.
- 遗传学重建以解决C SINE子家族 (C1和C2).
- 在HeLa细胞中进行功能测试,以测试子胚胎的多基化决定因素和转录组概况.
主要成果:
- 在子和子中发现了1.2至160万个C SINE副本,分为C1和C2.2,有两个子家族.
- C1 SINEs是具有功能性多基解信号的T+ SINEs,其前期为C2.2.
- 皮卡中没有C2 SINE,但在子和子中反转换活跃;C1变体在皮卡中表现出偶尔的活动.
- AATAAA动机和多聚胺通道是C1多基化最小的cis决定因素.
- 在子胚胎中,pol III的C SINE转录在16细胞阶段被激活.
结论:
- 在Lagomorpha中,C SINE家族在C1和C2亚家族之间显示出不同的进化轨迹和功能特征.
- 特定的序列元素决定了C SINE多化,突出显示了适应性进化.
- 由Pol III驱动的C SINE转录在发育过程中受到调节,始于早期胚胎发生.
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