精液扩展剂的克罗辛补充剂在延长储存期间保持冷雄羊精液质量
Vasiliki G Sapanidou1, Maria P Tsantarliotou2, Sophia N Lavrentiadou2
1Laboratory of Anatomy and Physiology of Farm Animals, Department of Animal Science, School of Animal Biosciences, Agricultural University of Athens, Iera Odos 75 Str., 11855 Athens, Greece.
Animals : an open access journal from MDPI
|March 14, 2026
概括
将抗氧化剂crocin添加到雄羊精液扩展器中,可以改善冷藏期间的精子质量. 克罗辛的1毫米度有效地保持了多达三天的运动性,活力和动力学参数.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物化学 生化学
背景情况:
- 雄羊精液易受寒冷冲击的影响,导致膜损伤.
- 由于反应性氧物种 (ROS) 的积累,冷却的公羊精液质量下降.
- 抗氧化剂可以减轻精液储存期间的氧化应激.
研究的目的:
- 调查克罗辛对冷藏公羊精液质量产生的保护作用.
- 评估克罗辛对精子参数和氧化应激标志物的影响.
- 为了确定最佳的crocin度延长精液的生命力.
主要方法:
- 雄羊精液在5°C下存储了3天,用0.5mM或1mM的crocin.在扩展器中.
- 评估了精子的运动性,活力,谷氨水平,热应激反应 (HSR) 蛋白质和亡.
- 每隔24小时进行统计分析.
主要成果:
- 克罗辛补充剂改善了精子运动性 (直到第一天),活力 (直到第二天) 和动力学参数 (直到第三天).
- 细胞内谷氨和Hsp70水平由crocin以剂量依赖的方式增加.
- 克罗辛显著抑制了亡,显示出抗氧化和细胞保护作用.
结论:
- 克罗辛作为一种强大的抗氧化剂和细胞保护剂,用于冷的公羊精液.
- 1毫米克罗辛在保存雄羊精子质量方面有效,冷藏保存长达三天.
- 克罗辛作为雄羊精液扩展剂的有价值添加剂,有望提高冷保存结果.
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