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AQP7通过线粒体活动保护玻璃化绵羊GV阶段卵细胞成熟

Yatian Qi¹, Wei Xia^1,2, Chenyu Tao^1,2

¹College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China.

Animals : an open access journal from MDPI

|March 14, 2026

概括

水素-7 (AQP7) 对于保护玻璃化绵羊卵细胞免受氧化压力至关重要. 维持AQP7功能可以保持氧化还原平衡和线粒体健康,增强卵细胞成熟和发育潜力.

科学领域:

生殖生物学生殖生物学
细胞生理学细胞生理学
低温生物学低温生物学

背景情况:

卵细胞玻璃化会诱导氧化应激,损害成熟和发育能力.
水素-7 (AQP7) 与细胞氧化还原调节有关,但其在冷保存卵细胞中的作用尚不清楚.

研究的目的:

研究AQP7在玻璃化和加热期间保护羊卵细胞中的作用.
为了确定AQP7是否保护细胞内氧化还原稳定和线粒体功能.

主要方法:

在生殖囊泡 (GV) 阶段,羊卵细胞被玻璃化并用AQP7抑制剂加热.
通过微注射进行了AQP7淘汰和过度表达.
评估了发育能力,成熟率和线粒体功能.

主要成果:

抑制AQP7破坏了氧化还原平衡和线粒体功能,显著降低了裂变和胚胎细胞形成率.
AQP7的淘汰进一步降低了成熟率,而过度表达部分恢复了它们.
针对线粒体的抗氧化剂MitoQ部分挽救了成熟率,这表明AQP7在减轻氧化应激方面的作用.

结论:

AQP7对于玻璃化绵羊卵细胞的成熟至关重要.
AQP7保护细胞内氧化还原稳定,防止线粒体功能障碍,并保持胚胎发育潜力.

关键词:

抗氧化剂是一种抗氧化剂.水族 7 7 水族 7 在体外成熟的成熟.线粒体中的线粒体.卵子细胞的玻璃化氧化应激是一种氧化应激.

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