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在玉米中识别候选耐热基因,通过结合链接和转录组分析

Mei Han^1,2, Xianfeng Yang^3,4, Jingfu Ma^3,4

¹College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

Plants (Basel, Switzerland)

|March 14, 2026

概括

全球变暖导致玉米的热应激,降低作物产量. 研究人员确定了与玉米耐热性相关的关键基因,为开发更适应气候变化的作物提供了途径.

科学领域:

植物遗传学和育种.
在作物中的非生物应激反应.
基因组学和转录基因组学

背景情况:

由于全球变暖,高温压力对玉米 (Zea mays L.) 的产量和质量构成重大威胁.
热应激会导致玉米的日晒,损害光合作用,导致产量损失.

研究的目的:

确定与玉米耐热性相关的定量特征位点 (QTL).
确定参与玉米对热应激反应的候选基因.

主要方法:

从耐热 (Zheng58) 和耐热 (HSBN) 玉米杂交系中开发出F2和F2:3种群.
在三个环境中进行了晒伤的表型鉴定,并使用10K SNP阵列对F2种群进行了基因型鉴定.
构建了基因图,并进行了转录组分析,以识别与QTLs同定位的差异表达基因 (DEGs).

主要成果:

在染色体1,4,5和8上确定了耐热度的8个QTL,解释了显著的表型变异.
在染色体1上发现了一个稳定的QTL (qHT1-2) 和一个主要的QTL (qHT1-3),其中qHT1-3解释了33.70%的变异.
发现了9262个DEGs,其中21个在QTL间隔内同定位,并确定了四个高度差异表达的候选基因 (Zm00001eb013260,Zm00001eb012720,Zm00001eb013600,Zm00001eb013100).

结论:

这项研究为培育耐热玉米品种提供了宝贵的遗传资源.
已识别的QTL和候选基因对于理解和改善玉米对热应激的抵抗力至关重要.

关键词:

在 QTL 映射中使用 QTL 映射.候选基因是指候选基因的基因.热应激是一种热应激.玉米玉米玉米是一种转录组分析转录组分析

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