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Updated: Mar 16, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

光纤干扰显微镜用于单分子水平上无标签监测癌细胞分泌.

Guoquan Wang¹, Yunli Peng¹, Guifeng Li¹

¹Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education of China, College of Optoelectronic Engineering, Chongqing University, Chongqing 400044, China.

Analytical chemistry

|March 14, 2026

概括

光纤干扰显微镜 (FOIM) 可实现无标签,高对比度单分子可视化. 这种技术为检测癌细胞分泌物和推进分子动力学分析提供了更高的灵敏度.

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Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

Published on: August 4, 2015

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Published on: August 4, 2015

科学领域:

生物物理学的生物物理.
光学显微镜的使用方法
分子诊断学分子诊断

背景情况:

单分子分析对于理解健康和疾病中的生物过程至关重要.
目前的无标签光学方法由于光热效应和信号对比而面临局限性.
需要先进的技术来持续监测分子动力学.

研究的目的:

开发一种新的无标签显微镜技术,用于单分子可视化.
提高在复杂环境中检测生物分子的灵敏度和特异性.
为了改善诊断,使分子动态的无标签监测成为可能.

主要方法:

光纤干扰显微镜 (FOIM) 的开发和应用.
在微纤维上利用自然缺陷产生的干扰模式.
用捕获抗体对光纤表面的功能化,用于有针对性的检测.

主要成果:

FOIM实现了单个分子的无标签,高对比度可视化.
空间解析的干扰模式揭示了由单个分子诱导的局部相变.
在复杂的细胞外环境中,证明了对癌细胞分泌物的高度特定检测.

结论:

与传统方法相比,FOIM提供了显著的灵敏度增强.
该技术表现出高的目标亲和力和对干扰分子的抵抗力.
FOIM是用于无标签的分子动态监测和下一代诊断的强大工具.