发现了伴侣蛋白质依赖的草类生物合成
Riley S Carter1, Sangeetha Ramesh2, Hamada Saad3
1Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
ACS chemical biology
|March 14, 2026
概括
这项研究揭示了依赖合作伙伴蛋白质的新型草类生物合成途径,揭示了独特的宏循环结构和酶修饰. 这些发现扩大了已知的核糖体合成和翻译后修饰 (RiPPs) 的多样性.
科学领域:
- 生物化学和分子生物学
- 自然产品的发现自然产品的发现
- 生物信息学是一种生物信息学.
背景情况:
- Graspetides 是通过核糖体合成和翻译后修改的 (RiPPs),具有复杂宏循环结构的潜力.
- 许多预测的草生物合成基因集群 (BGCs) 包含未被发现的定制酶,能够修改脚架.
- 关于草类生物合成的现有知识缺乏对伴侣蛋白质依赖和新型循环化策略的例子.
研究的目的:
- 调查草类生物合成基因集群 (BGCs) 的新型定制酶和生物合成途径.
- 发现新的草类结构并了解它们独特的生物合成,包括伴侣蛋白参与.
- 加强生物信息工具,以识别多种草类和相关的定制酶.
主要方法:
- 更新了RODEO生物信息工具以识别草和相关的裁酶.
- 为大规模生物信息分析生成了超过20,000个预测草类的数据集.
- 通过体外生化试验优先鉴定和特征化了两种新型的草基因基因与保存的同时发生的蛋白质.
主要成果:
- 发现了第一个依赖伙伴蛋白的草酸生物合成的例子.
- 在草类中确定了前所未有的循环化5-氧化类部分.
- 罗萨利提德具有三种相互锁定的宏拉克顿链接和五个重复的动机和一个新的基化步骤的珊瑚.
- 证明了罗萨利胺合成酶的活性和稳定性取决于配净合作伙伴蛋白质.
结论:
- 这项研究显著扩大了已知的草生物合成谱,揭示了新的结构特征和酶机制.
- 这些发现强调了伙伴蛋白在某些复杂RiPPs的生物合成中的关键作用.
- 增强的生物信息学方法和体外表征提供了未来发现各种草虫的基础.
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