与微化集成的超声波:结构修改以改善红胡种子蛋白质分离物的体外可消化性,功能性和抗氧化特性
Muhammad Faisal Manzoor1, Muhammad Waseem2, Tazeddinova Diana3
1Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, Foshan, China; School of Food Science and Engineering, South China University of Technology, Guangzhou, China.
Ultrasonics sonochemistry
|March 14, 2026
概括
微缩和超声处理显著改善了红胡种子蛋白分离物 (RPSPI) 的稳定性和技术功能性质. 这提高了未充分利用的蛋白质来源的价值.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 红胡种子是一种未充分利用的农业副产品,富含蛋白质.
- 蛋白质分离物通常需要加工以提高其用于食品应用的功能性质.
研究的目的:
- 从红胡种子 (RPSPI) 中提取蛋白质分离物.
- 研究微化 (MN) 和超声波 (US) 治疗单独和联合对RPSPI的影响.
- 评估对RPSPI的物理化学,结构,技术功能和生物活性性质的影响.
主要方法:
- 从红胡种子中提取蛋白质.
- 微化 (15,000rpm3-6分钟) 和超声波 (720W,40kHz10-15分钟) 处理的应用.
- 分析了粒子大小,泽塔潜力,分子结构 (自由SH,疏水性,光,FTIR),热性质 (DSC,XRD),技术功能性质 (溶解性,乳化,泡,水/油容量),抗氧化活性 (ABTS,DPPH,OH清除) 和体外可消化性.
主要成果:
- 结合MN (6分钟) 和US (10分钟) 处理显著降低了颗粒大小和增加了泽塔潜力,提高了RPSPI的稳定性.
- 结构分析显示,自由SH含量增加,表面水性,随机线圈和α螺旋体含量增加,光和结晶性降低,表明部分展开和改变分子间相互作用.
- 技术功能性质 (溶解度,乳化活性,泡能力,水/油保持能力) 显著改善,度降低.
- 结合治疗显著增强了抗氧化活性和体外蛋白质消化能力.
结论:
- 非热处理,特别是结合微化和超声波,有效地修改红胡种子蛋白质隔离结构.
- 这些修改大大改善了RPSPI的技术功能和生物活性特性.
- 这项研究强调了利用红胡种子蛋白作为食品应用中增值成分的潜力.
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