BtuJ1是Bacteroidota中的表面暴露的B12结合蛋白,作为细胞外维生素储存器,增强健身的功能
Javier Abellon-Ruiz1, Raul Pacheco-Gomez2, Jessica Watts2
1Biosciences Institute, The Medical School, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
Journal of molecular biology
|March 14, 2026
概括
肠道中的细菌菌体利用BtuJ1,一种表面脂蛋白,有效地获得维生素B12. 这种蛋白质保持了易于获得的维生素B12池,对于营养有限的环境中的细菌健康至关重要.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 维生素B12的获取对于Bacteroidota肠道开始性健康至关重要.
- 吸收依赖于涉及BtuB和BtuG的外膜 (OM) 综合体,BtuH也在其中发挥作用.
- 辅助OM蛋白在营养获取中发挥着不同的作用.
研究的目的:
- 为了描述BtuJ1,一个额外的表面脂蛋白参与了Bacteroides thetaiotaomicron中维生素B12的吸收.
- 在细菌获得维生素B12的背景下阐明BtuJ1的功能和结构.
主要方法:
- 功能测试以确定BtuJ1对维生素B12和胺胺的结合亲和力.
- BtuJ1.1 的结构特征.
- 在体外和体内实验来评估B12转移动态的实验.
- 在不同的B12条件下BtuJ1丰度的量化.
主要成果:
- BtuJ1以低纳米分子亲和力结合维生素B12和胺,在B12有限的条件下增强适应性.
- 不管B12的可用性如何,BtuJ1是B.theta中最丰富的B12运输成分,不管B12的可用性如何.
- 在充足的条件下,BtuJ1储存维生素B12,以便在耗尽期间以后转移到BtuBG系统.
结论:
- BtuJ1扩展了在Bacteroidota中辅助OM蛋白的已知功能.
- 在竞争激烈的肠道环境中,BtuJ1代表了确保维生素B12的复杂策略.
- 这些发现凸显了 Bacteroidota 在获取必需营养素方面所使用的复杂机制.
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