组织溶解的分子景观揭示了肝脏调节网络和代谢媒介,这些媒介是的料效率的基础
Wenxin Zhang1, Fangren Lan1, Yuejie Han2
1State Key Laboratory of Animal Biotech Breeding, Frontier Science Center of Molecular Design Breeding, China Agricultural University, Beijing 100193, China; National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100193, China; Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
这项研究揭示了产母的料效率的分子基础,显示了肝脏网络,脂质代谢和脂肪沉积之间的联系. 这些发现为提高家禽料效率提供了见解.
科学领域:
- 禽类遗传学和生理学
- 代谢学和转录学.
- 动物育种与营养 动物育种与营养
背景情况:
- 剩余料摄入量 (RFI) 对家禽料效率至关重要,影响可持续生产.
- 了解RFI的遗传和分子基础,特别是其与产母脂肪沉积的联系,是不完整的.
研究的目的:
- 确定产母的料效率的分子特征.
- 阐明长期产卵期间料效率和脂肪沉积之间的分子关系.
主要方法:
- 在248只母中进行了整体基因组再测序,多组织转录组学和代谢组学.
- 采用遗传学协会,cis-eQTL映射,跨omics集成和机器学习.
- 利用基于肝细胞的功能测试来评估100周RFI (100wRFI) 的候选基因和代谢物.
主要成果:
- 确定了与100wRFI相关的基因组位点,并优先考虑因果基因/代谢物.
- PCCB成为一个关键的多组织候选者,形成一个以肝脏为中心的轴与PE{18:0/20:4}{8Z,11Z,14Z,17Z)).
- 干扰PCCB改变了肝脏的脂质生成,氧化还原状态和炎症;LPI(18:1) 在体外促进了脂质的积累.
结论:
- 描绘了老母的料效率的组织解析的分子景观.
- 突出显示了连接脂质代谢,平衡和料效率的肝脏调节网络.
- 强调了料效率和脂肪沉积之间的分子联系,为未来的研究提供了框架.
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