PIN1通过诱导细胞衰老和激活干扰素反应途径来抑制膀癌的进展
Linlin Zhang1, Cong Chen2, Wei Huang2
1Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Urology,The Second Affiliated Hospital of Shandong First Medical University, Tai'an 271000, Shandong, China.
Cellular signalling
|March 14, 2026
概括
乙-乙 cis-trans 异构酶 NIMA 相互作用 1 (PIN1) 作为膀癌的瘤抑制剂,通过诱导衰老和激活干扰素信号来抑制进展. 低PIN1表达预测生存率较低,并提供治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 膀癌 (BCa) 是一种常见的恶性瘤,复发率高.
- 在膀瘤发生过程中,基-基 cis-trans 异构酶 NIMA 相互作用 1 (PIN1) 的作用尚不清楚.
- 在其他癌症中,PIN1被认为是瘤基因,但其在BCa中的功能尚不清楚.
研究的目的:
- 研究PIN1在膀癌中的表达,功能和分子机制.
- 为了确定PIN1是否在BCa.中充当瘤抑制剂或瘤基因.
- 评估PIN1作为预后生物标志物和潜在的治疗点.
主要方法:
- 单细胞RNA测序 (scRNA-seq) 用于分析瘤微环境异质性.
- 生物信息分析包括差异表达,生存相关性和衰老相关性.
- 通过免疫组织化学,西斑和qRT-PCR验证PIN1表达的验证.
- 在体外测试扩散,亡,迁移,入侵和衰老.
- 在体内异种移植小鼠模型来评估瘤生长和衰老.
- RNA测序和途径丰富分析以阐明分子机制.
主要成果:
- 在BCa组织的恶性上皮细胞中,PIN1显著下调.
- 低PIN1表达与整体和无进展生存率差相关.
- 过度表达PIN1可以抑制膀癌细胞的增殖,迁移和入侵,同时促进细胞亡和衰老.
- 提升PIN1的调节抑制了瘤的生长,并增加了体内衰老.
- PIN1激活了干扰子反应通路,包括IFNAR1,IRF7和IRF9.
- 干扰素信号调解PIN1的瘤抑制作用.
结论:
- 在膀癌中,PIN1作为一种新的瘤抑制剂起作用.
- PIN1通过诱导衰老和激活干扰素信号传递来抑制膀癌的进展.
- PIN1是一种有价值的预后指标,也是膀癌的潜在治疗点.
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