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工程 Bacillus thuringiensis Cyt1Aa作为Cry1A类虫杀虫蛋白的替代受体发挥作用

Nathaly Alexandre do Nascimento¹, Mary Carmen Torres-Quintero¹, Blanca Ines García-Gómez¹

¹Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.

Pesticide biochemistry and physiology

|March 14, 2026

概括

科学家们设计了一种多功能Cyt1Aa毒素,作为Bacillus thuringiensis (Bt) Cry1A毒素的替代受体. 这种新的方法提高了Bt毒素的有效性,并提供了一种潜在的策略,以克服转基因作物的害虫耐药性.

科学领域:

生物化学生物化学
分子生物学分子生物学
昆虫学昆虫学是一门学科.

背景情况:

转基因作物表达Bacillus thuringiensis (Bt) Cry1杀虫蛋白对于控制类害虫至关重要.
昆虫对Bt作物的广泛耐药性威胁到这种害虫控制技术的长期有效性.
杀虫剂Cyt1Aa毒素可以通过作为替代受体来协同杀蚊Cry蛋白.

研究的目的:

为了设计Cyt1Aa毒素以作为类鸟Cry1Ab和Cry1Ac毒素的替代受体.
调查工程Cyt1Aa在克服对Bt作物的害虫耐药性的潜力.

主要方法:

通过将scFv73抗体的互补性确定区域 (CDR3) 引入暴露循环中,从而创建Cyt1Aa-73杂交物来改造Cyt1AaA59C.
评估了Cyt1Aa-73杂交物与Cry1Ab和Cry1Ac毒素的结合.
评估了由Cyt1Aa-73杂交物诱导的Cry1Ab和Cry1Ac的体外寡合化.
测试了Cyt1Aa-73杂交物对敏感和耐药Plutella xylostella幼虫的增强毒性.

主要成果:

这三种Cyt1Aa-73杂交蛋白有效结合Cry1Ab和Cry1Ac,在体外诱导毒素的寡合化.
Cyt1Aa-73杂交蛋白显著增强了Cry1Ab和Cry1Ac对易受感染的Plutella xylostella的毒性.
一种混合体,Cyt1Aa-73 L7,增加了对抗抗性Plutella xylostella菌株的Cry1Ac毒性,具有ABCC2转运器突变.

结论:

改造的Cyt1Aa可以作为类鸟Cry1A毒素的替代受体.
这种工程方法显示出作为一种新的工具来打击对Bt作物的昆虫耐药性的承诺.
这些发现提供了一种潜在的策略,以恢复基于Bt的农业害虫防治的有效性.

关键词:

细菌 thuringiensis 这种疾病.哭泣中的毒素细胞毒素是细胞毒素的毒素.杀虫剂蛋白质是一种杀虫剂.电阻电阻电阻电阻电阻电阻协同作用协同作用协同作用

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