相关实验视频

Updated: Mar 16, 2026

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

乙胆结合蛋白5调节狼蜘蛛的细胞外亡,作为一种牺牲性ROS清洁者

Huihui Zhang¹, Jingting Wang², Yao Tan³

¹Key Laboratory of Biohazard Monitoring and Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot 010010, China; Key laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.

Pesticide biochemistry and physiology

|March 14, 2026

概括

研究人员发现了一种新的方法,蜘蛛使用乙胆结合蛋白5 (AChBP5) 来保护细胞免受损伤. 这种蛋白质起到抵御氧化压力和杀虫剂的作用,突出了新的细胞外亡机制.

科学领域:

*分子生物学和毒理学研究.
*研究亡的保存和物种特异性机制.

背景情况:

* 亡调节涉及保存的细胞内途径,但细胞外机制不太了解.
* 细胞外亡调节通常涉及特定物种的基因组,阻碍跨物种的比较.

研究的目的:

* 在狼蜘蛛Pardosa pseudoannulata中识别新的细胞外亡机制.
* 研究乙胆结合蛋白5 (AChBP5) 在细胞应激反应和亡中的作用.

主要方法:

*对5个ACHBP基因的转录分析,以对杀虫剂和活性氧物种 (ROS) 的反应.
*使用培养细胞进行功能性测试,以评估ACHBP5作为抗氧化应激的细胞外缓冲剂的作用.
*RNA干扰 (RNAi) 沉默ACHBP5表达并评估蜘蛛对压力的敏感性.

主要成果:

* AChBP5在蜘蛛脂肪体中高度表达,其表达是由杀虫剂和ROS诱导的.
* AChBP5作为细胞外牺牲缓冲剂,以度依赖的方式保护细胞免受ROS诱导的损伤.
*沉默ACHBP5显著增加了蜘蛛对氧化应激和杀虫剂暴露的敏感性.

结论:

* AChBP5调解了蜘蛛中一种新的细胞外亡机制,作为抗氧化应激的保护性缓冲.
*像ACHBP5这样的血统特异性基因有助于细胞外亡调节,并提供对化学压力因素的防御.
* 这一发现提供了关于蜘蛛弹性和适应环境挑战的见解.

关键词:

在 AChBP 中,细胞灭亡 (apoptosis) 是一种死亡的过程.杀虫剂是一种杀虫剂.氧化应激是一种氧化应激.狼蜘蛛是什么?狼蜘蛛是什么?

更多相关视频

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

Detection and Isolation of Apoptotic Bodies to High Purity

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

相关概念视频

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

The Extrinsic Apoptotic Pathway

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...

Phagocytosis of Apoptotic Cells

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...

Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...

Apoptosis

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...

Autophagic Cell Death

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...