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转录因子CncC/Maf通过调节Locusta migratoria中的UGT392C1来调节伊米达克洛普里德的敏感性

Chen Chen¹, Peng Shi², Haihua Wu³

¹Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; School of life science, Shanxi University, Taiyuan, Shanxi 030006, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, Shanxi 030006, China; Shanxi Key Laboratory of Nucleic Acid Biopesticides, Shanxi University, Taiyuan, Shanxi 030006, China.

Pesticide biochemistry and physiology

|March 14, 2026

概括

通过控制排毒基因,CncC/Maf通路调节虫对杀虫剂的敏感性. 这项研究确定了LmUGT392C1作为这一过程中的关键基因,对伊米达克洛普里德排毒至关重要.

科学领域:

昆虫学昆虫学是一门学科.
分子生物学分子生物学
生物化学生化学

背景情况:

异生体转录因子 (XFTs),包括CncC和Maf,对于调节排毒酶基因和调节杀虫剂敏感性至关重要.
了解这些调节途径对于管理农业害虫的抗虫剂耐药性至关重要.

研究的目的:

阐明CncC/Maf信号通路在Locusta migratoria中的杀虫剂敏感性和排毒中的作用.
为了确定参与杀虫剂代谢的CncC/Maf调节的特定下游基因.

主要方法:

基因表达分析 (LmCncC和LmMaf的组织局部化).
使用RNA干扰 (RNAi) 和CRISPR-Cas9基因编辑的功能研究.
进行比较的转录学,以识别下游目标.
酶抑制试验 (使用硫芬拉来抑制UGTs).
双露西法酶记者基因测定以确认促进体激活.

主要成果:

在消化组织和脂肪体中,lmCncC和lmMaf的表达很高.
击败lmCncC/lmMaf增加了对deltamethrin和imidacloprid的敏感性.
三个尿素二酸盐-葡萄糖酶转移酶 (UGT) 基因被确定为下游目标.
抑制UGT活动增加了对杀虫剂的敏感性.
证实LmUGT392C1是一种由CncC/Maf调节的关键排毒基因,对伊米达克洛普里德耐药性至关重要.

结论:

CncC/Maf-LmUGT392C1调节轴在调节L. migratoria.昆虫杀虫剂敏感性方面发挥着至关重要的作用.
这一途径对于伊米达克洛普里德的排毒至关重要.
研究结果为开发克服抗虫剂耐药性或识别新耐药性机制的策略提供了洞察力.

关键词:

这是CncC/Maf.伊米达克洛普里德 (Imidacloprid) 是一种龙迁徙者 (英语:Locusta migratoria) 是一种迁徙的动物.这是一种UDP-glucuronosyltransferase (UGT) 酶.

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