相关实验视频
Updated: Mar 16, 2026

08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
8.4K
在成年神经干细胞群体内进行Notch3信号传递
Sara Ortica1, Miguel Martinez Herrera1, Louis Degroux1
1Institut Pasteur, Université Paris Cité, CNRS UMR3738, Zebrafish Neurogenetics Unit, F-75015 Paris, France.
Nature communications
|March 15, 2026
概括
在成年大脑中,Notch3信号控制神经干细胞 (NSC) 的静止和干性. 它的水平是由deltaA和jagged1b连接体的空间模式,影响NSC命运决策.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 干细胞生物学 干细胞生物学
背景情况:
- 在成人大脑中,Notch3信号传递对于维持神经干细胞 (NSC) 静止和干性至关重要.
- 控制Notch3信号水平的精确机制及其对NSC命运的直接影响仍然不清楚.
研究的目的:
- 直接测量成年斑马鱼大脑NSC中的Notch3信号动态.
- 研究Notch3信号水平与NSC静止和干性之间的关系.
- 阐明deltaA (dla) 和jagged1b (jag1b) 连接体在Notch3信号模式中的作用.
主要方法:
- 在斑马鱼成年telencephalon NSCs中Notch3细胞内片段 (N3ICD) 核转移的量化.
- 在NSC中的Notch3信号,连接体表达 (dla,jag1b) 和干性因子 (Sox2) 的现场分析.
- 对jag1b水平进行实验操作,以评估其对Notch3信号和NSC属性的影响.
主要成果:
- 诺奇3信号水平与NSC静止和茎状直接相关.
- 诺奇3信号是空间模式,高信号环绕N3ICD-低细胞表达dla.
- Jag1b激活了Notch3信号,并保持了Sox2的表达,而它的减少改变了信号变异,而不会破坏空间模式.
结论:
- 诺奇3信号集成了侧向抑制 (通过dla) 和侧向诱导 (通过jag1b),以调节成年NSC静止和干性.
- 这些信号通路控制NSC命运决定的时空动态.
- 了解Notch3信号提供了对成年神经发生和干细胞维护的见解.
相关概念视频
Notch Signaling Pathway
6.8K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.8K
Notch Signaling Pathway
6.4K
6.4K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.6K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K
Determination
21.3K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
21.3K
Hedgehog Signaling Pathway
10.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
10.3K
Neurulation
47.0K
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...
47.0K

