标准化口腔微生物群采样QPCR:对营养状况的方法和探索性见解
Karina Mendes1, Ana T P C Gomes1, Cristina Maria Mendes Resende2
1Center for Interdisciplinary Research in Health (CIIS), Faculty of Dental Medicine (FMD), Universidade Católica Portuguesa, Estrada da Circunvalação, 3504-505, Viseu, Portugal.
Scientific reports
|March 15, 2026
概括
非刺激的唾液是使用定量聚合酶链反应 (qPCR) 量化口腔细菌的最一致的方法. 这种可重复的非侵入性方法非常适合青少年健康和营养研究中的微生物组研究.
科学领域:
- 微生物学 微生物学
- 人体生理学 人体生理学
- 分子生物学分子生物学
背景情况:
- 精确的口腔微生物群量化需要标准化的样本采集.
- 定量聚合酶连锁反应 (qPCR) 是细菌计数的一个关键方法.
研究的目的:
- 评估和确定使用qPCR的最一致的口服样本采集方法,用于青少年的细菌量化.
- 通过比较青少年的细菌形状和身体组成来评估最佳方法的生物适用性.
主要方法:
- 从青少年那里收集了未经刺激的唾液,脸和生物膜样本.
- 使用qPCR量化总细菌 (16S rRNA基因),Bacillota和Bacteroidota族的数量.
- 进行了探索性分析,将细菌数量与身体组成参数相关联.
主要成果:
- 与脸和生物膜 (p <0.05) 相比,未刺激的唾液在细菌量化中产生了最低的变异性.
- 在唾液和生物膜之间发现了中等的相关性,唾液和脸拭片之间的关联很弱.
- 虽然超重/肥胖青少年的细菌数量有较高的趋势,但这些差异在统计学上并不显著.
结论:
- 非刺激的唾液是一种经过验证的,可复制的,非侵入性的,具有成本效益的生物流体,用于通过qPCR量化口腔微生物群.
- 这些发现支持唾液在营养和代谢健康研究中的大规模,转化或护理点微生物组评估中的实用性.
- 口腔细菌与人体组成之间的特定群体关联突出了唾液在健康研究中的潜力.
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