在转移高分辨率质谱学中通过化学反应进行软电离,用于临床呼气分析
Camille Roquencourt1,2, Elodie Lamy2,3, Nicolas Hunzinger2,3
1Hôpital Foch, Exhalomics®, 40, Rue Worth, 92150, Suresnes, France.
Analytical and bioanalytical chemistry
|March 15, 2026
概括
通过转移中的化学反应 (SICRIT) 软电离加上高分辨率质谱 (HRMS) 提供了一种可复制的方法来分析呼气中的挥发性有机化合物 (VOC),从而推进呼吸学研究.
科学领域:
- 分析化学 分析化学
- 代谢学 代谢学 代谢学
- 生物技术是生物技术.
背景情况:
- 呼气分析是一种非侵入性方法,用于检测与代谢和病理状态相关的挥发性有机化合物 (VOC).
- 呼吸分析的传统方法在范围和灵活性上可能受到限制.
研究的目的:
- 评估通过转移中的化学反应 (SICRIT) 接口的软电离的可行性,可重复性和化学覆盖,并与高分辨率质谱学 (HRMS) 结合用于临床呼气分析.
- 评估SICRIT-HRMS对全面的人类呼吸代谢组概况的潜力.
主要方法:
- 使用SICRIT-HRMS实时分析40名健康志愿者的呼气.
- 采用信号处理,包括特征对齐,过和复合注释,使用人类新陈代谢数据库 (HMDB) 和人类呼吸学数据库 (HBDB).
主要成果:
- 可复制检测到604个光谱特征,其中80%匹配HMDB,56%匹配HBDB.
- 识别了基和半挥发性氨基酸,其中位数可重复性在呼气中为13%,在个体中共相似度为0.98.
- 路径丰富分析强调了氨基酸和脂质代谢;与性别相关的25种VOC和与禁食持续时间相关的6种VOC.
结论:
- SICRIT-HRMS提供了人体呼吸代谢组的高分辨率,实时分析,具有出色的可重复性和广泛的化学覆盖.
- 这项技术是呼吸学研究的强大工具,并为未来的翻译诊断应用提供了希望.
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