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从裂谷热病病毒RNA开发参考材料

Changwoo Park¹, Il-Hwan Kim², Seil Kim^3,4,5

¹Biometrology Group, Division of Biomedical Metrology, Korea Research Institute of Standards and Science, Daejeon, Republic of Korea.

Virology journal

|March 15, 2026

概括

新裂谷热病毒 (RVFV) RNA参考材料 (RMs) 现已可用. 这些RM可确保对RVFV进行准确的分子检测,改善对这一重大公共卫生威胁的诊断和控制.

科学领域:

病毒学病毒学
分子诊断学分子诊断
计量学计量学是一门学科.

背景情况:

裂谷热病毒 (RVFV) 是一种主要在非洲发现的重要病原体.
分子诊断测试,如核酸放大测试,对于RVFV检测至关重要.
目前,RVFV核酸的计量认证参考材料是不可用的,这阻碍了精确的分子测试.

研究的目的:

为裂谷热病毒 (RVFV) 开发和描述测量学认证的RNA参考材料.
为RVFV分子诊断试验的性能评估和质量评估提供必要的工具.

主要方法:

在实验室中转录的RNA,包括RVFV基因组的L,M和S段,用于创建参考材料.
逆转录滴滴数字PCR (RT-ddPCR) 用于在开发的参考材料中绝对量化病毒RNA拷贝数.

主要成果:

开发的RVFV RNA参考材料 (KRISS 111-10-537,538和539) 呈现出极好的同质性,瓶子之间的副本数量变化在4.0%至6.7%之间.
这些参考材料表现出强大的稳定性,在4°C下保持长达14天,在-80°C下保持长达12个月的稳定性.
量化RNA拷贝数在7.82×104到2.51×106拷贝/μl之间.

结论:

新开发的RVFV RNA参考材料为病毒基因拷贝数提供了准确的参考值.
这些材料有望减少RVFV分子测试中的假阳性和假阴性.
这些参考材料的可用性将有助于改善谷热的诊断和控制.

关键词:

反转录滴滴数码PCR的反转录方式裂谷热病病毒病毒参考材料是参考材料的参考材料.

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