由NAT10催化的CTCFβ-基基化调节MHC-II基因表达
Rui Zhou1, Shirui Li1, Siyi Jiang1
1School of Life Sciences, Chongqing University, 401331, Chongqing, China.
The international journal of biochemistry & cell biology
|March 16, 2026
概括
CCCTC结合因子 (CTCF) 蛋白经历NAT10的β-基基化,影响其染色素结合,并影响MHC-II基因表达. 这一发现将代谢信号与通过CTCF修饰的基因调节联系起来.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 代谢调节 代谢调节 代谢调节
背景情况:
- 在基因表达和基因组组织中,CCCCTC结合因子 (CTCF) 是至关重要的.
- 在CTCFβ-基基化 (Kbhb) 的生物学作用仍然在很大程度上是未知的.
研究的目的:
- 为了研究CTCFβ-基基基化过程的生物学意义.
- 为了探索CTCF和NAT10之间的相互作用在β-基基化的背景下.
主要方法:
- 研究了CTCF与N-乙转移酶10 (NAT10) 的相互作用.
- 在U2OS细胞中通过NAT10在lysine 202 (K202) 中分析了CTCFβ-hydroxybutyrylation.
- 评估BHB治疗和代谢变化 (STZ,禁食) 对细胞系和小鼠脏中的CTCF局部化和MHC-II基因表达的影响.
主要成果:
- CTCF与NAT10相互作用,NAT10在K202.2中调解其β-基基基化.
- 通过NAT10介导的β-基基化减少并改变了与染色质结合的CTCF的局部.
- 减少染色质结合的CTCF与改变的MHC-II基因表达相关,特别是在BHB水平增加的情况下.
结论:
- 通过NAT10进行CTCFβ-基基化影响CTCF染色体结合和局部化.
- CTCFβ-基基化在调节MHC-II基因表达方面发挥着重要作用.
- 这项研究揭示了代谢信号,染色体动力学和通过CTCF修饰的基因调节之间的新鲜联系.
关键词:
B细胞系 拉吉 (Raji) 细胞系在CTCF中,CTCF是指CTCF.在MHC-II中,MHC-II是最常见的.在 NAT10 中,NAT10 包含了NAT10.链毒素 (STZ) 链毒素β-基乙酸盐 (BHB) 是一种β-基基基化 (Kbhb) 的方法更多相关视频
12:09Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
Published on: February 28, 2019
10.4K
11:06Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
Published on: September 20, 2017
6.6K
相关概念视频
Antigen Processing Pathways
2.8K
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
2.8K
Antigens Involved in Adaptive Immunity
1.8K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
1.8K
Receptor Downregulation in MVBs
2.9K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.9K
Regulation of Expression at Multiple Steps
1.5K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K
Histone Modification
16.9K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.9K
NF-κB-dependent Signaling Pathway
10.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
10.2K
