HPLC-Orbitrap-MS用于果汁和食补充剂中B族维生素的确定

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Lucia Bartella^1,2, Fabio Mazzotti^1,2, Ilaria Santoro^1,2

¹Dipartimento di Chimica e Tecnologie Chimiche, Università Della Calabria, Rende, CS, Italy.

Journal of mass spectrometry : JMS

|March 16, 2026

概括

这项研究提出了一种强大的HPLC-ESI-MS方法,用于量化水果汁和补充剂中的七种B族维生素. 经过验证的技术确保了复杂的食品矩阵中准确的维生素分析.

科学领域:

分析化学分析化学
食品科学食品科学食品科学
生物化学生物化学

背景情况:

由于复杂的矩阵,准确的食品维生素分析具有挑战性.
维生素B是对人类健康至关重要的微量营养素.
现有的方法可能缺乏用于同时确定的灵敏度或特异性.

研究的目的:

开发和验证七种B族维生素的同时分析方法.
应用B族维生素在果汁和食补充剂中的量化方法.
评估方法性能,包括准确性,精度和灵敏度.

主要方法:

使用了Orbitrap MS技术与高性能液体染色学 (HPLC) 和电喷射电离 (ESI).
分析了胺, рибофлавин, 尼亚, 潘托酸, 氧化物, 生物素, 和叶酸.
用于果汁的直接稀释和补充剂的溶剂提取,与逆相染色学和全扫描MS检测.

主要成果:

实现了极好的线性 (R2>0.998) 和良好的精度 (RSD<15%).
证明了可接受的准确性 (补充剂的96%-112%,果汁的~100%).
成功应用于真实样本,证实了标签声明和维生素含量,新鲜果汁的度更高.

结论:

经过验证的HPLC-ESI-MS方法提供了七种B族维生素的准确和敏感量化.
该方法适用于对B族维生素的常规分析,这些维生素存在于各种食品表格中,如果汁和补充剂.
研究结果强调了新鲜果汁和商业果汁之间的维生素含量差异.

关键词:

维生素B组的维生素包括:食补充剂食补充剂果汁果汁果汁果汁果汁高分辨率的质谱仪.定量测定定量测定量测定量测定量测量量量测量量量测量量量测量量量测量量量测量量量测量量量测量量量测量量量测量量量测量量量量测量量量测量量量量测量量量量测量量量量测量量量

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