相关实验视频
Updated: Mar 17, 2026

13:07
One-channel Cell-attached Patch-clamp Recording
Published on: June 9, 2014
25.6K
皮埃佐1通道调节C2C12细胞中小细胞外囊的释放
Bernareggi Annalisa1, Zhang Wen Ru2, Sanchez-Sanchez Laura2
1Department of Life Sciences, University of Trieste, Trieste, Italy.
Journal of cellular physiology
|March 16, 2026
概括
使用Yoda1激活PIEZO1通道,可增强小细胞外囊泡的释放,并改变它们在骨肌细胞中的载荷. 这些囊泡促进肌管的形成,突出显示PIEZO1.
科学领域:
- 细胞生物学 细胞生物学
- 肌肉生理学 肌肉生理学
- 细胞外囊泡 细胞外囊泡
背景情况:
- PIEZO1通道是机械敏感的离子通道,对细胞功能至关重要.
- 它们在骨肌肉再生中的作用是公认的,但机制尚不清楚.
- 小小的细胞外囊泡调解细胞间的通信,对于组织修复至关重要.
研究的目的:
- 为了研究PIEZO1对肌性C2C12细胞中小细胞外囊泡释放的影响.
- 描述PIEZO1激活对囊泡内容和功能的影响.
- 探索PIEZO1在骨肌肉前体细胞通信中的作用.
主要方法:
- 用PIEZO1激动剂Yoda1.1.治疗肌细胞和肌管.
- 通过超离心,西式涂抹,纳米跟踪和蛋白质组分析,分离和描述释放的小细胞外囊泡.
- 评估囊泡介导对髓管形成的影响.
主要成果:
- Yoda1治疗显著增加了细胞外小囊的释放,并改变了神经管中的蛋白质荷载,但不是神经质细胞.
- 来自Yoda1治疗细胞的小型细胞外囊泡促进了具有不同容量的肌管形成.
- 囊泡中的PIEZO1表达在髓管中增加,这表明细胞类型特定的生物发生.
结论:
- PIEZO1的激活控制了小细胞外囊泡的释放和载荷在肌性前体.
- PIEZO1通过细胞外囊介导的通信在骨肌肉再生中发挥着重要作用.
- 这些发现阐明了PIEZO1在骨肌肉生理病理学中的功能.
相关概念视频
Overview of Secretory Vesicles
9.8K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.8K
Mechanically-gated Ion Channels
8.0K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
8.0K
Feedback Regulation of Calcium Concentration
4.1K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
4.1K
Pinching-off of Coated Vesicles
4.4K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.4K
Fusion of Secretory Vesicles with the Plasma Membrane
19.3K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
19.3K
GPCRs Regulate Adenylyl Cylase Activity
8.1K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
8.1K

