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差异表达的循环微RNA的计算分析和前列腺癌中关键基因的识别

Anveshika Manoj¹, Shweta Kumari¹, Gautam Prasad¹

¹Department of Biochemistry, King George's Medical University, Lucknow, Uttar Pradesh India.

Indian journal of clinical biochemistry : IJCB

|March 16, 2026

概括

这项研究确定了九种微RNA (miRNA) 及其向基因,这些基因与前列腺癌有关. 其中8种miRNA显示出作为前列腺癌诊断和预后的新生物标志物的潜力.

科学领域:

生物信息学是一种生物信息学.
分子生物学分子生物学
在瘤学瘤学.

背景情况:

前列腺癌产生大量数据集,包括组织和体液中的失调的微RNA (miRNA).
在的方法提高了查失调的miRNAs的准确性和可访问性.

研究的目的:

为了识别微RNA并探索它们的信使RNA (mRNA) 相互作用在前列腺癌的发展.
研究已识别的miRNAs作为诊断和预后生物标记物的潜力.

主要方法:

使用GEO-2R对GEO数据集GSE112264进行分析,以识别显著上下调节的miRNAs.
目标组分析用于绘制miRNA-目标基因相互作用的地图,并构建交互网络.
功能丰富分析 (DAVID 6.8,GSEA) 用于KEGG,Reactome和基因本体学-生物过程 (GO-BP) 途径.

主要成果:

确定了190个显著的miRNA,其中9个miRNA针对20个独特的基因,这些基因对前列腺癌的生存和扩散至关重要.
发现了两种新的miRNAs (miRNA-520a-3p和miRNA-550a-3p),涉及前列腺癌的发病因子.
突出显示了8种具有非侵入性诊断和预后生物标志物应用潜力的miRNA.

结论:

已识别的miRNA及其向基因为前列腺癌进展提供了洞察力.
八种miRNA值得进一步研究,因为它们是前列腺癌的潜在非侵入性生物标志物.
目标基因分析可以指导前列腺癌管理的新疗法的开发.

关键词:

生物信息学分析循环中的微RNA.在GEO数据集中,GEO数据集包括:基因丰富是一种基因丰富.前列腺癌是什么意思前列腺癌是什么意思目标基因是目标基因.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...