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首次应用QuEChERS-GC-MS分析用于在人类脂肪组织中检测多环芳香碳化合物

Alice Franchin¹, Elena Gregoris², Luca Sorarù¹

¹Ca' Foscari University of Venice, Department of Environmental Sciences, Informatic and Statistics, via Torino 155, Venice (Mestre), Veneto 30172, Italy.

|March 16, 2026

概括

这项研究开发了一种新的方法来测量人类脂肪组织中有毒的多环芳 (PAHs). 经过验证的技术在人体样本中成功检测出像烯这样的PAH,有助于生物监测工作.

科学领域:

环境化学环境化学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

生物监测通过测量污染物度来评估人类对危险物质的暴露.
多环芳 (PAH) 是有毒的,脂性环境污染物,在脂肪组织中生物积累.
在人类脂肪组织中分析PAH存在挑战,原因是矩阵复杂性和采样侵入性.

研究的目的:

开发和验证可靠的分析方法来量化人类脂肪组织中有毒的PAH.
为了解决生物监测复杂矩阵的差距,例如人类脂肪对PAH暴露的生物监测.
通过推进污染物检测方法,为PRIN 2022 PNRR项目"INSYDE-HU"做出贡献.

主要方法:

使用QuEChERS (快速,轻松,廉价,坚固,安全) 提取技术进行样本制备.
采用气相色谱与质谱学 (GC-MS) 结合用于敏感和特定的PAH分析.
在应用到人类脂肪组织之前,验证了使用牛脂肪作为替代基质的方法.

主要成果:

成功开发并验证了14种PAH的方法,该方法的检测和量化极限较低 (分别为0.114.13和0.3013.76 ng g-1).
在人类脂肪组织样本中检测到,,和.
在人体样本中的量化烯在2.344.88 ng g-1.1的范围内.

结论:

开发的QuEChERS-GC-MS方法在复杂的人类脂肪组织中有效确定有毒PAH.
这项研究提供了在这个具有挑战性的矩阵中用于PAH分析的少数验证方法之一.
这些发现增强了人类脂肪组织中脂性污染物的生物监测能力.

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