基于RuBisCO的CO2固定改善了Corynebacterium glutamicum中的谷氨酸酸的产生
Aiying Wei1,2, Jingui Liu1, Yulin Tang1
1College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Frontiers in bioengineering and biotechnology
|March 16, 2026
概括
工程 *Corynebacterium glutamicum* 提高二氧化碳用于生物生产的利用. 补充本地代谢与二氧化碳固定通路显著提高了谷氨酸的生产和碳效率.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 生物技术是生物技术.
背景情况:
- 为化学生物生产利用二氧化碳对于减轻排放和实现碳中和至关重要.
- 卡尔文-本森-巴什姆 (CBB) 循环为二氧化碳固定提供了一个自然模型.
- 将异质基酶 (PRK) 和RuBisCO引入微生物工厂是利用二氧化碳的一个有前途的战略.
研究的目的:
- 为了改造*Corynebacterium glutamicum*以提高二氧化碳的吸收和生物生产.
- 评估两种代谢策略:替代原生途径与用异构的二氧化碳固定途径补充它们.
- 通过促进工程和适应性实验室进化,优化工程途径.
主要方法:
- 在C. glutamicum*中引入异构的RuBisCO-PRK通路.
- "替代"和"补充"代谢策略的比较.
- 在二氧化碳压力下使用促进器工程和适应性实验室进化 (ALE) 的优化.
主要成果:
- "替代"战略削弱了增长和生产.
- "补充"策略显著提高了代谢性能.
- 优化品种的谷氨酸度达到196.78g/L,产量增加,葡萄糖消耗减少.
结论:
- 补充本地代谢与二氧化碳固定分流是优于替换在*C. glutamicum*的重要途径.
- 这项研究为开发碳负微生物细胞工厂提供了一个可扩展的蓝图.
- 异质二氧化碳同化与工业发酵的成功整合表明了可持续生物生产的可行方法.
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