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Updated: Mar 18, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

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协同CRISPR-Cas9宿主工程和酶进化用于Vibegron Pyrrolidine中间体的酶选择性合成

Jinping Bao1,2,3, Xiaozhi Ju1,2, Han Zhang1,2,4

  • 1State Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Chinese Academy of Sciences, Tianjin 300308, China.

Organic letters
|March 16, 2026
PubMed
概括

这项研究介绍了一种新型生物催化方法,用于生产一个关键的vibegron中间体,使用胺基还原酶 (IRED) 酶工程. 开发的全细胞系统为性罗利丁合成提供了可扩展和高效的途径.

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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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