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昆虫细胞的N-Glycoengineering用于三天线N-Glycan生物合成

Hiroyuki Kajiura^1,2, Naokuni Nishiguchi^1,3, Reimi Lai Sang Sawada-Choi^1,3

¹International Center for Biotechnology, The University of Osaka, 2-1 Yamada-oka, Suita, Osaka, 565-0871, Japan.

Scientific reports

|March 17, 2026

概括

工程昆虫细胞生产复杂的三天线N-甘氨酸包括引入特定的糖转移酶和抑制内源酶. 这项研究通过增强Spodoptera frugiperda Sf9细胞中的N-糖化,促进生物制药生产.

科学领域:

生物技术是生物技术.
葡萄糖生物学葡萄糖生物学
生物制药生产生物制药生产

背景情况:

昆虫细胞为生物制药提供了高生产率,但产生了不良的昆虫特有的N-糖.
类似哺乳动物的N-甘氨酸是生物制药产品的首选,需要对昆虫细胞糖化途径进行工程.

研究的目的:

为了工程 Spodoptera frugiperda Sf9 昆虫细胞,以高效地生产三天线 N-glycans.
开发一种实用的昆虫细胞平台,用于生产适合生物制药的复杂N-甘氨酸.

主要方法:

在昆虫细胞中识别并引入人类N-乙葡萄糖胺基转移酶IV (GNTIV).
与昆虫衍生的葡萄糖转移酶 (GNTI,GNTII) 共同表达的GNTIV,以增强三天线N-葡萄糖合成.
研究了内源性葡萄糖酶在N-葡萄糖修剪中的作用.

主要成果:

仅引入人类GNTIV就导致了三天线N-甘氨酸的低水平.
三种N-乙葡萄糖胺基转移酶的同时表达显著增加了三天线N-甘氨酸生物合成.
发现内源性甘油酶可以切割N-甘氨酸,因此需要抑制它们以有效生产.

结论:

在昆虫细胞中高效的三天线N-甘氨酸生物合成需要外源性甘氨基转移酶表达和内源性甘氨基酸酶的抑制.
这种工程化Sf9细胞平台为生物制药制造中生产复杂N-甘氨酸提供了可行的替代方案.
进一步的优化可能涉及针对性基因的淘汰或特定的葡萄糖酸酶的淘汰.

关键词:

这种N-Acetylglucosaminyl转移酶IV的作用.这是一种N-乙hexosaminidase.通过N-Glycosylation进行了N-Glycosylation.GlcNAc 的扩展名为 GlcNAc 昆虫细胞是昆虫的细胞.

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