Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

3.6K
3.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.8K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.8K
Improving Translational Accuracy02:07

Improving Translational Accuracy

15.4K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
15.4K
Regulated mRNA Transport02:22

Regulated mRNA Transport

3.5K
3.5K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.8K
5.8K
Reporter Genes02:11

Reporter Genes

13.7K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

A distal promoter and aberrant splicing enable canonical translation of out-of-frame proteins in Huntington's disease.

bioRxiv : the preprint server for biology·2025
Same author

Current practices in the study of biomolecular condensates: a community comment.

Nature communications·2025
Same author

Rapid expansion and specialization of the TAS2R bitter taste receptor family in amphibians.

PLoS genetics·2025
Same author

Sequence programmable nucleic acid coacervates.

bioRxiv : the preprint server for biology·2024
Same author

Repeat-associated non-AUG translation induces cytoplasmic aggregation of CAG repeat-containing RNAs.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same journal

Chromosome condensation mechanically primes the nucleus for mitosis.

The EMBO journal·2026
Same journal

NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

The EMBO journal·2026
Same journal

Assembly of the catalytic module and the rotor of human ATP synthase.

The EMBO journal·2026
Same journal

Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC.

The EMBO journal·2026
Same journal

Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.

The EMBO journal·2026
Same journal

Cellular assembly and functional resilience of the mammalian RNA exosome.

The EMBO journal·2026
查看所有相关文章

相关实验视频

Updated: Mar 18, 2026

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
06:18

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

Published on: April 26, 2019

6.5K

当RNA脱离脚本时:确保转基因表达中的转录忠实性

Rachel Anderson1,2, Christalyn Ausler1, Ankur Jain3,4

  • 1Whitehead Institute for Biomedical Research, 455 Main Street, Cambridge, MA, 02142, USA.

The EMBO journal
|March 17, 2026
PubMed
概括
此摘要是机器生成的。

质粒可以引起意想不到的RNA转录和蛋白质,扭曲研究结果. 用RNA-seq验证转基因表达和共享等离子体序列对于准确的科学结论至关重要.

关键词:
异常的拼接 异常的拼接在Codon优化过程中,隐秘的促进者 隐秘的促进者塑体 塑体 塑体处理RNA处理RNA处理

更多相关视频

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
10:49

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Published on: March 6, 2020

6.5K
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

10.0K

相关实验视频

Last Updated: Mar 18, 2026

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
06:18

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

Published on: April 26, 2019

6.5K
Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
10:49

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

Published on: March 6, 2020

6.5K
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
09:30

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

10.0K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物技术是生物技术.

背景情况:

  • 等离子体是分子生物学中克隆,过度表达和基因操纵的重要工具.
  • 从等离子体产生的RNA转录的真实性通常是假设而不是验证.
  • 等离子体骨干序列,标签和优化区域可能包含意想不到的监管元素.

研究的目的:

  • 突出塑体导致错误实验结果和解释的例子.
  • 检查等离子体关联表达器件的机制和影响.
  • 提出减轻这些文物和提高实验严谨性的策略.

主要方法:

  • 发表文献的审查,记录了等离子体诱导的表达文物.
  • 在常见的等离子体组件中分析潜在的隐秘促进器和拼接部位.
  • 讨论例证研究,说明未被检测到的表达错误的后果.

主要成果:

  • 识别了许多已发表的例子,在这些例子中,从标准的等离子体使用中产生的意外转录和蛋白质.
  • 展示了这些文物如何导致对基因功能和实验结果的误解.
  • 强调了这些错误有可能传播到重要的研究成果和临床应用中.

结论:

  • 假设完美的等离子体转录忠实度经常是错误的,可能会损害研究完整性.
  • 实施用于检测和预防等离子体相关工件的策略对于可靠的科学发现至关重要.
  • 倡导社区标准,包括存放完整的等离子体序列和使用RNA-seq进行转录验证至关重要.