相关实验视频

Updated: May 6, 2026

Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation

Published on: February 15, 2018

早产婴儿的克洛斯特里迪亚会代谢母乳的寡糖类,抑制病原生物,调节有机体中的肠功能

Jonathan A Chapman¹, Andrea C Masi¹, Lauren C Beck¹

¹Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, UK.

Nature microbiology

|March 17, 2026

概括

某些Clostridium物种,特别是那些缺乏PfoA毒素的物种,可以代谢母乳寡糖化物 (HMO). 这些微生物产生有益的化合物,可以通过抑制有害细菌和减少炎症来支持健康的婴儿肠道发育.

科学领域:

微生物学微生物学
婴儿营养婴儿营养
肠道微生物组研究研究

背景情况:

婴儿肠道微生物组的发展受到母乳和人乳寡糖化物 (HMO) 的影响.
HMO代谢主要研究在Bifidobacteria中,但其他能够利用HMO的物种在很大程度上是未知的.
结核性肠球炎是早产婴儿的病理,可以受到肠道微生物组合的影响.

研究的目的:

调查HMO利用的微生物物种范围,超出早产婴儿肠道中的Bifidobacteria.
描述Clostridium物种对婴儿肠道环境的代谢潜力和功能影响.
探索特定的Clostridium菌株在促进健康的肠道微生物群和减轻病理方面的潜力.

主要方法:

用基因组,蛋白质组和代谢组分析来识别和描述HMO代谢细菌.
进行了体外培养实验,以评估代谢物生产和微生物相互作用.
使用结肠细胞系和早产的肠道器官的实验进行,以评估Clostridium物种及其超级生物的功能作用.

主要成果:

克洛斯特里物种,包括缺乏perfringolysin O (PfoA) 的Clostridium perfringens菌株,被确定为HMO代谢器.
与Bifidobacterium物种相比,这些Clostridium物种产生了更多的有益代谢物,例如短链脂肪酸和托催化剂.
来自缺乏PfoA的C. perfringens的无细胞超级生物抑制了病原生物的生长,促进了开始性Bifidobacteria的生长,抑制了有机体的炎症,对结肠细胞无毒.

结论:

这项研究扩大了已知的HMO代谢微生物的范围,包括某些Clostridium物种.
缺乏PfoA的Clostridium perfringens菌株表现出有益的特性,包括产生促进健康的代谢物和调节肠道环境.
这些发现表明,特定的C. perfringens菌株可能在支持健康的婴儿肠道发育方面发挥作用,并且可能是未来治疗干预的候选人.

更多相关视频

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Published on: November 14, 2018

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

相关概念视频

Development of Human Microbiota

Development of Human Microbiota

The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from...

Development of the Oral Microbiota

Development of the Oral Microbiota

The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...

Functions of the Gut Microbiota

Functions of the Gut Microbiota

The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...