非共价核桃蛋白水解剂-多复合物的形成和表征:多多样性在功能调节中的作用
Chao Yan1, Huiwen Guan1, Xuchun Zhu1
1Key Laboratory of Geriatric Nutrition and Health, School of Food and Health, Beijing Technology and Business University, Beijing 100048, China.
Journal of agricultural and food chemistry
|March 17, 2026
概括
利用核桃副产品的价值创造了可持续的功能成分. 核桃蛋白水解盐 (WPH) 与多的复合物增强了溶解性,抗氧化能力和生物可访问性.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 可持续化学 可持续化学
背景情况:
- 核桃加工产生了富含蛋白质和多的副产品.
- 这些副产品的价值化是可持续的功能成分开发的关键.
- 了解核桃蛋白水解盐 (WPH) 和多之间的相互作用是必不可少的.
研究的目的:
- 研究WPH与特定的核桃多 (酸,酸,酸,咖啡酸,甲) 之间的非对应复合物的形成和特性.
- 评估复杂化对WPH和多的功能性质和体外消化能力/生物可访问性的影响.
主要方法:
- 形成WPH-多复合物的过程.
- 谱分析 (光火) 用于研究结合相互作用.
- 使用循环二元论分析WPH的结构变化.
- 测量功能性质 (可溶性,表面张力,乳化活性,抗氧化能力).
- 酶抑制试验 (α-氨基酶,α-葡萄糖酶).
- 风病学测量. 风病学测量. 风病学测量.
- 在体外消化和多生物可访问性研究.
主要成果:
- 证实了非共价复合体的形成,具有主导的疏水相互作用 (除了由静电力驱动的WPH-epicatechin).
- 复杂化诱导了WPH的结构变化,降低了β-sheet,增加了β-turn含量.
- 所有复合物都表现出增强的溶解性,减少的界面张力和改善的乳化活性.
- WPH-酸显示出最高的抗氧化能力;WPH-酸显示出最强的α-氨酶和α-葡萄糖酶抑制.
- 风病学分析表明,由于更强的网络形成,粘度和粘弹性增加.
- 试验室消化显示蛋白质消化能力降低,但显著提高了多的生物可访问性.
结论:
- 可以有效地形成WPH-多复合物,增强关键的功能性质.
- 这些综合体为利用核桃副产品制成多功能,可持续的成分提供了一个有希望的途径.
- 复杂化调节了消化能力和生物可访问性,突出了食品和营养药品行业的潜在应用.
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