相关实验视频
Updated: Oct 13, 2026
08:07
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
旁分泌的骨源性衰老分泌组与中国台湾人脑类器官中空间模式化的细胞外基质重塑及生物力学改变相关
Chanul Kim1, Luke Daniel Ofria2, Hossein Shaygani3
1Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Stem cells (Dayton, Ohio)
|October 12, 2026
Abstract:
衰老正日益被视为一种全身性过程，然而外周衰老细胞释放的信号如何加速大脑衰老的机制仍不明确。本研究通过将人脑类器官长期暴露于衰老骨细胞的分泌组中，探究骨组织来源的外周衰老信号如何重塑脑组织结构。我们结合了基于光纤的高空间分辨率干涉测量纳米压痕技术与转录组学及免疫荧光分析，发现在不同检测方法中存在显著差异的力学特征：完整类器官的表观模量降低，而在冷冻切片样本中则出现模量升高的局部区域。这种空间异质性的生物力学重塑伴随着细胞外基质、炎症及衰老相关通路的上调，以及神经发育和突触基因网络的抑制。结果表明，长期旁分泌暴露于衰老骨细胞来源的分泌组与人脑组织中局部的细胞外基质相关重塑及机械脆弱性相关，揭示了外周细胞衰老可能在衰老过程中导致脑区脆弱性的潜在机制。本研究为初步探索性结果，使用单一iPSC细胞系和单一批次的条件培养基，各实验条件下重复数为1至6次，力学分析属探索性质。
相关概念视频
01:40Bone Remodeling
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
01:31Osteoclasts in Bone Remodeling
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
01:29Bone Disorders
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...