相关实验视频
Updated: Aug 3, 2026

11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
概括
斯肉瘤病毒RNA序列在瘤DNA中存在于低频率,一些病毒序列在较高度中发现. 正常的小胚胎DNA也显示了与病毒RNA的部分同质性.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 罗斯肉瘤病毒 (RSV) 是一种致癌逆转录病毒.
- 了解病毒基因组融入宿主DNA对于癌症研究至关重要.
研究的目的:
- 为了调查来自病毒诱导的肉瘤的DNA中的Rous肉瘤病毒 (RSV) 基因组序列的存在和频率.
- 为了比较瘤DNA中的病毒序列分布与正常小胚胎DNA.
主要方法:
- 来自布拉格RSV菌株的71SRNA与来自病毒诱导的肉瘤的多余DNA之间的杂交的动态分析.
- 量化瘤和正常DNA样本中每个细胞的病毒序列频率.
主要成果:
- 大多数病毒基因组序列在瘤DNA中被检测到,每个细胞的平均频率非常低.
- 大约三分之一的病毒序列被发现具有更高的平均频率 (每细胞50-100个副本).
- 正常的小胚胎DNA含有部分同源于病毒RNA的一小部分序列,与瘤DNA不同.
结论:
- 病毒基因组序列被整合到罗斯肉瘤病毒诱导的瘤的细胞DNA中.
- 病毒序列的可变频率表明了整合或表达的潜在差异.
- 正常小DNA中的同源序列表明可能存在内源性病毒元素或先前存在的细胞反应.
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In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
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Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
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