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Updated: Sep 16, 2026

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
11:01

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Published on: September 25, 2016

在二次葡萄糖来源上培养哺乳动物细胞的生长

J G Rheinwald, H Green

    Cell
    |August 1, 1974
    PubMed
    概括

    No abstract available in PubMed .

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    Cell Culture01:21

    Cell Culture

    Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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