抗皮cillin的Haemophilus influenzae B型具有TEM型β-乳糖酶,但对皮cillin的透性障碍很小
Lancet (London, England)
|March 29, 1975
概括
抗白素抗性Haemophilus influenzae B型菌株具有TEM型β-乳糖酶,由于细胞透性增加,抗性较低. 这一发现影响了对这种病原体抗生素耐药性的理解.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
背景情况:
- 最近出现的抗西林抗性Hemophilus influenzae B型 (Hib) 是一个令人担忧的问题.
- 在Hib分离物中,已经确定了TEM型β-乳酸酶,这是一种常见的格拉姆阴性细菌的耐药机制.
研究的目的:
- 研究hemophilus influenzae B型抗素耐药性的机制.
- 为了比较TEM型β-lactamase对H. influenzae中的青素耐药性的影响,与其他格兰阴性细菌相比.
主要方法:
- 分析了来自美国和德国的5个AMPICILLIN耐药H.influenzaeB型的临床分离物.
- 描述β-乳糖酶活性和确定青素耐药水平.
- 在H. influenzae和Escherichia coli中对青素的细胞透性的比较分析.
主要成果:
- 所有五种H. influenzaeB型分离物都含有TEM型β-乳酸酶.
- 与大肠杆菌相比,H. influenzae对青素的细胞透性明显更高.
- 获得TEM型β-乳糖酶导致单个H. influenzae细胞的青素耐药性比E. coli细胞的增强不那么明显.
结论:
- 流感菌对青素的透性增加,减轻了TEM型β-乳糖酶在单个细胞水平上所产生的耐药性.
- 在大型注射剂中观察到高水平的耐药性,这表明了注射剂效应.
- 单个细胞中微妙的耐药性增加可能解释了对安培耐药H. influenzae型B的延迟识别,并需要进一步的临床研究.
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