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哺乳动物核糖体与MS2菌体RNA的结合揭示了编码溶解功能的重叠基因

J F Atkins, J A Steitz, C W Anderson

|October 1, 1979

概括

哺乳动物的核糖体与菌体MS2RNA结合,转化一个重叠的溶解基因. 该基因中的特定突变 (op3) 影响菌体复制酶的产生和溶解.

科学领域:

分子生物学分子生物学
病毒学病毒学
遗传学是一个遗传学.

背景情况:

菌体MS2RNA含有重叠的基因,在外套蛋白质基因的下游有一个溶解基因.
哺乳动物的核糖体可以在外套蛋白质基因中的AUG编码中启动翻译,产生溶解多.
菌素f2中的op3突变会影响溶解和复制酶的产生.

研究的目的:

描述菌体MS2中的重叠溶解基因.
调查op3突变在菌体基因表达和功能中的作用.
为了比较原生细胞和真核细胞系统之间的核糖体结合和翻译启动.

主要方法:

使用哺乳动物和大肠杆菌无细胞系统进行RNA导向的蛋白质合成.
合成的多的部分氨基酸序列分析.
在菌体f2.2中对op3突变的遗传分析.

主要成果:

哺乳动物的核糖体有效地从细菌的MS2RNA中翻译出75个氨基酸溶解聚.
该op3突变是合成酶cistron中的C-to-U过渡,影响溶解和复制酶的产生.
与哺乳动物系统相比,大肠杆菌无细胞系统产生了很少量的溶解聚.

结论:

重叠的溶解基因被哺乳动物核糖体高效地翻译.
op3突变破坏了溶解启动区,影响了溶解和复制酶合成.
在大肠杆菌和哺乳动物系统之间存在溶解启动区的核糖体识别的差异.

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