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内源和外源的摩洛尼小鼠白血病病毒的整合部位

H van der Putten, E Terwindt, A Berns

|September 1, 1979

概括

莫洛尼小鼠白血病病毒的整合部位在基因倾向和受感染的小鼠之间有所不同. 白血病的发展涉及Moloney前病毒DNA重新整合到瘤组织中的新部位.

科学领域:

病毒学病毒学
遗传学遗传学是一种遗传学.
在瘤学瘤学.

背景情况:

鼠类白血病病毒 (MLVs) 是已知的导致小鼠白血病的逆转录病毒.
了解MLV前病毒集成对于研究白血病产生至关重要.
遗传倾向和病毒感染影响整合模式.

研究的目的:

在Balb/Mo和Balb/c小鼠基因组中对Moloney和AKR MLV前病毒集成部位进行表征.
为了研究病毒整合和莫洛尼病毒诱导的白血病之间的关系.
为了比较遗传传染病与获得感染的整合模式.

主要方法:

为Moloney和AKR MLVs准备特定的cDNA探针.
限制酶消化 (Eco RI) 和南方斑点分析小鼠基因组DNA.
在正常和瘤组织中对前病毒DNA整合部位的分析.

主要成果:

在Balb/Mo小鼠中,基因传染的Moloney预病毒被发现在一个特定的16 x 10(6) 达尔顿的Eco RI片段.
这种特定的片段在新生儿感染莫洛尼病毒的Balb/c小鼠中不存在.
两种小鼠模型中的白血病发生都涉及Moloney病毒序列重整到瘤组织中的新染色体位点.
在两种小鼠线的12 x 10 ((6) 达尔顿Eco RI片段中始终发现AKR前病毒序列,没有证据表明新的整合部位.

结论:

在生殖线中占据的病毒整合部位在产后感染时不一定占据.
莫洛尼病毒诱导的白血病与前病毒性DNA重新整合到新的基因组位置有关.
遗传传染的AKR前病毒似乎稳定,并没有通过融合部位的改变直接参与Moloney诱导的白血病发生.

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