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酵母组织基因H2A和H2B的分离

L Hereford, K Fahrner, J Woolford

|December 1, 1979

概括

酵母组织基因H2A和H2B在平分体基因组内存在两个副本. 这些基因对由非同源DNA分开,并以不同的方式转录,其他蛋白质也被编码.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
酵母生物学的酵母生物学

背景情况:

基质子是真核生物中DNA包装的关键蛋白质.
了解基因组织组织的基因组织组织,为基因组调节提供了洞察力.
以前的研究已经描述了一些酵母组织基因的特征.

研究的目的:

分析酵母基因组基因H2A和H2B的基因组组织.
为了确定这些基因组基因的复制数和排列.
为了研究转录方向和周围的序列.

主要方法:

酵母 DNA 序列的克隆.
克隆基因的序列分析.
进行比较基因组学以评估同源性.

主要成果:

在平分类酵母基因组中确定了H2A和H2B基因对的两个副本.
在每个副本中发现了大约700 bp的H2A和H2B之间的非同类间隔DNA.
确定两个基因副本在基因组中分离35-60kb.
在两个副本中观察到H2A和H2B的分离转录.
发现了这些等离子体编码的其他非希斯蛋白的存在.
在H2A/H2B附近没有发现H3和H4基因的证据.

结论:

酵母基因组包含H2A和H2B基因的特定重复安排.
非同类间隔DNA表明基因拷贝之间的潜在调节差异.
不同转录是这种基因组基因对的保存特征.
这种组织意味着酵母体中 histone 生产的复杂调节机制.

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