概括
布莱克斯莱亚三胞胎 (-) 菌株从 (+) 菌株制造的前体中合成三胞胎酸C性激素. 这种合成的酶似乎是构成性的,因为它们不受actidione的抑制.
科学领域:
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
- 生殖生物学 生殖生物学
背景情况:
- 布莱克斯莱亚trispora是一种真菌,以其在三酸生物合成中的作用而闻名.
- 三酸作为性激素起作用,在真菌中调解交配和繁殖.
- 合成途径涉及不同交配菌株 (+ 和 -) 的不同贡献.
研究的目的:
- 调查Blakeslea trispora中的三酸C的起源和合成.
- 为了确定 (+) 菌株合成的前体的性质.
- 为了确定在 (-) 菌株中负责三酸C合成的酶是否可诱导或构成.
主要方法:
- 培养Blakeslea trispora (+) 和 (-) 菌株的培养方法.
- 从 (+) 菌株的培养基中提取非酸性前体,使用.
- 从 (-) 菌株中分离的前体中测试actidione对三酸合成的影响.
主要成果:
- 布莱克斯莱亚三胞胎的 (-) 菌株从 (+) 菌株提供的前体中合成三胞胎酸C.
- 这些前体是非酸性化合物,可通过形式提取分离.
- 艾克蒂迪翁并没有抑制从这些分离的前体中合成三酸C.
结论:
- 在Blakeslea trispora的 (-) 菌株中合成三酸C时,利用了 (+) 菌株的前体.
- 在 (-) 菌株内,参与三酸C合成的酶是构成性酶,这是由于没有被actidione抑制而表明的.
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