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玉米中的酒精脱酶:对酶活性的遗传控制

Science (New York, N.Y.)

|November 13, 1970

概括

发现了一种新的基因 - - 酒精脱酶调节器 (Adhr(r) - - 控制玉米酒精脱酶活性. 它与Adh1位点有关,但影响酶活性,而不是电荷.

科学领域:

* 遗传学和分子生物学
* 植物生物化学植物生物化学

背景情况:

* 酒精脱酶 (Adh) 在植物新陈代谢中至关重要.
* 玉米中的Adh1位点控制酶的充电和迁移.
* 了解酶活性的基因调节是必不可少的.

研究的目的:

* 鉴定和表征一种新型基因,该基因调节玉米皮中的酒精脱酶活性.
* 确定这个新基因与已知的Adh(1) 位点之间的关系.

主要方法:

* 基因分析以确定Adhr (r) 基因.
*对不同等位基组组合的酶活性水平的检查.
*链接分析以确定基因接近性.

主要成果:

*发现了一种新的基因,Adhr,控制Adh活性.
* Adhr (r) 不是Adh (h) 的等位基因,但与Adh (r) 有关,距离它大约有17个交叉单位.
* Adh (r) (N) 基因基因对 Adh (l) (S) 和 Adh (l) (F) 产物的活性相同.
* 在cis和trans配置中,Adh (r) (L) 的等位基因降低了Adh (l) (S) 产物的活性.

结论:

*一个独特的基因位点,Adhr(r),调节玉米皮中的酒精脱酶活性.
* 这种调节基因独立于Adh (1) 位点运作,该位点决定了酶的电泳性质.
* Adh (r) (l) 基因基因基因表现出对 Adh 异酶表达的复杂调节作用.

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