概括
一种新型的热友性,酸友性 prokaryote,Thermoplasma acidophila,在一个自我加热的煤垃圾堆中被发现. 这种缺乏细胞壁的有机体在极端温度和酸性条件下壮成长,扩大已知的真菌体息地.
科学领域:
- 微生物学 微生物学
- 极端爱好研究 极端爱好研究
- 细胞生物学 细胞生物学
背景情况:
- Prokaryotes 通常具有细胞壁用于结构完整性.
- 菌质是一种独特的细菌群,其特点是缺乏细胞壁.
- 极端环境是各种微生物的息地,它们具有独特的适应能力.
研究的目的:
- 从一个自我加热的煤垃圾堆中分离和描述一种新型的 prokaryotic 生物.
- 确定孤立生物体的形态,生理和遗传特性.
- 调查这项发现对已知的菌等离子体相关生物息地的影响.
主要方法:
- 从煤炭废弃物中分离原核生物.
- 电子显微镜用于细胞壁缺失的确认.
- 对于赫索胺的化学测定.
- 用vancomycin进行增长抑制测定.
- 基因谷氨酸加氨酸 (GC) 含量分析.
- 跨温度和pH范围的生长研究.
主要成果:
- 隔离一个缺乏细胞壁的热友,酸友 prokaryote.
- 电子微图和生物化学测试证实没有细胞壁.
- 低DNAGC含量和芽生殖表明与真菌质有关.
- 在59°C和pH值为1-2的环境下,增长最好,温度和pH值的耐受性范围已定义.
- 拟定名称: 热等离子体酸性.
结论:
- 热等离子体酸性是一种独特的极端性,明显缺乏细胞壁.
- 生物体的特征将其置于或与菌等离子体血统相关.
- 这一发现扩大了对质菌发生的已知的环境范围.
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