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在MSV转化G8-124细胞中的MSV特异的亚基因组mRNA

D J Donoghue, P A Sharp, R A Weinberg

|May 1, 1979

概括

研究人员在MSV转化细胞中发现了一种新的3.1 kb亚基因组RNA. 这种RNA含有5'和3'序列,可能表达MSV特定信息,包括与转换相关的元素.

科学领域:

分子生物学分子生物学
病毒学病毒学
癌症研究癌症研究

背景情况:

鼠肉瘤病毒 (MSV) 的转变涉及复杂的遗传机制.
了解病毒RNA物种对于阐明致癌过程至关重要.

研究的目的:

来自MSV转化细胞的细胞内亚基因组RNA的结构和潜在功能的特征.
调查这种新型RNA物种的起源和组成.

主要方法:

用MSV和MuLV特定的cDNA探针对RNA:cDNA异质复合体的电子显微镜.
分析RNA组成,包括5'和3'衍生序列.

主要成果:

确定了3.1 kb的亚基因组RNA,包括0.4 kb的5'-衍生序列和2.7 kb的3'-衍生序列.
3'-衍生序列含有剩余的MuLV聚和获得的MSV细胞序列.
MSV基因组保留了MuLV env序列,但没有检测到具有拼接env序列的亚基因组RNA.

结论:

鉴定到的3.1 kb的MSV亚基因组RNA很可能是信使RNA,可能参与表达3'-编码的MSV信息.
这种RNA可能在MSV介导的细胞转化中发挥作用.
需要进一步的研究来确定这种亚基因组RNA的翻译产物和精确功能.

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