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在体外肌肉生成过程中,小肌肉细胞的mRNA群体的变化

B M Paterson, J O Bishop

|November 1, 1977

概括

在肌肉发育 (肌体发生) 过程中,小胚胎肌肉培养显著增加了特定信使RNA (mRNA) 序列的度. 这些丰富的mRNA分子编码关键的肌肉蛋白质,如肌肉蛋白和肌肉蛋白.

科学领域:

分子生物学分子生物学
发展生物学发展生物学
遗传学是一个遗传学.

背景情况:

肌体生成涉及复杂的基因表达调节.
了解mRNA种群为细胞分化提供了洞察力.

研究的目的:

为了分析mRNA序列的复杂性和丰富性,在小肌形成过程中.
为了确定在肌肉发育过程中增加的特定mRNA序列.

主要方法:

在不同发育阶段,从小胚胎肌肉培养物中分析mRNA种群.
量化mRNA序列的复杂性和丰富性.
无细胞蛋白质合成试验.
与DNA序列类型的相关性 (重复性与单拷贝性).

主要成果:

预流和阻塞培养有约17,000个mRNA序列.
肌纤维细胞培养显示约2500个丰富的序列和6个非常丰富的序列 (15,000个副本/核).
高度丰富的序列在premyogenic和阻塞培养物中明显较低.
丰富的mRNA与肌肉特异性蛋白质 (肌肉蛋白,动蛋白,肌肉蛋白) 的合成相关.
20-30%的丰富的mRNA是从中度重复的DNA转录的.

结论:

肌体生成的特点是特定的mRNA丰度显著增加.
这些丰富的mRNA编码主要的肌肉结构蛋白质.
重复和单复制DNA都会在肌体发生过程中为mRNA群体做出贡献.

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