概括
研究人员发现了多个复制的小鼠乳腺瘤病毒相关序列的复制品,这些序列集成到Mus musculus和Mus cervicolor的基因组中. 这些序列存在于许多染色体上,与正常组织相比,在瘤细胞中没有升高.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 病毒学 病毒学
背景情况:
- 鼠内甲型粒子 (A粒子) 是在小鼠中发现的内源性逆转录病毒元素.
- 这些序列的基因组组织和整合模式尚未完全理解.
研究的目的:
- 调查与小鼠内A型颗粒相关联的RNA序列的存在和基因组集成.
- 确定这些序列在不同小鼠组织和细胞系中的重复频率和染色体定位.
主要方法:
- 来自Mus musculus和Mus cervicolor的高分子重量核DNA的A粒子RNA序列的3H-cDNA探针的混合.
- 性糖糖梯度沉积,以评估DNA的整合.
- 在现场杂交以确定染色体定位.
- 在严格的条件下,与其他物种 (老鼠,仓鼠,豚鼠) 的DNA进行杂交研究.
- 直接杂交的多A) RNA与细胞DNA,以确认转录的存在.
主要成果:
- 在各种Mus musculus菌株和Mus cervicolor菌株的核DNA中检测到A粒子相关序列的多个副本 (1050-1800个单 haploid基因组).
- 在BALB/3T3细胞中观察到更少的副本 (450个);在瘤和正常组织中,重复频率相似.
- 序列被整合到小鼠基因组中,位于区域度的多个染色体上,在其他动物物种中显示出较低的同质性.
结论:
- 这项研究证实了小鼠基因组中A粒子相关序列的多个副本的广泛集成.
- 这些序列稳定整合并分布在许多染色体位置.
- 这些发现提供了对小鼠基因组内源逆转录病毒元素组成的见解.
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